Detection of Tiger Podoplanin Using the Anti-Cat Podoplanin Monoclonal Antibody PMab-52

Shinji Yamada, Shunsuke Itai, Yoshikazu Furusawa, Masato Sano, Takuro Nakamura, Miyuki Yanaka, Saori Handa, Kayo Hisamatsu, Yoshimi Nakamura, Masato Fukui, Hiroyuki Harada, Takuya Mizuno, Yusuke Sakai, Satoshi Ogasawara, Takeshi Murata, Hiroaki Uchida, Hideaki Tahara, Mika K. Kaneko, Yukinari Kato

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Podoplanin (PDPN) is expressed in type I alveolar cells of lung but not in type II alveolar cells. PDPN is also known as a specific lymphatic endothelial cell marker because PDPN is not expressed in vascular endothelial cells. PDPNs of several animals have been characterized using specific anti-PDPN monoclonal antibodies (mAbs): PMab-1, PMab-2, PMab-32, PMab-38, PMab-44, and PMab-52 for mouse, rat, rabbit, dog, bovine, and cat PDPNs, respectively. In this study, we investigated the possible crossreaction between these anti-PDPN mAbs and tiger PDPN. Flow cytometry and western blot analyses revealed that the anti-cat PDPN mAb PMab-52 (IgM, kappa) reacted with tiger PDPN, which is overexpressed in Chinese hamster ovary-K1 cells. Using immunohistochemical analysis, type I alveolar cells of the tiger lung were strongly detected by PMab-52. These results indicate that PMab-52 may be useful for the detection of tiger PDPN.

Original languageEnglish
Pages (from-to)224-228
Number of pages5
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume37
Issue number5
DOIs
Publication statusPublished - 2018 Nov

Keywords

  • PDPN
  • PMab-52
  • tiger podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Yamada, S., Itai, S., Furusawa, Y., Sano, M., Nakamura, T., Yanaka, M., Handa, S., Hisamatsu, K., Nakamura, Y., Fukui, M., Harada, H., Mizuno, T., Sakai, Y., Ogasawara, S., Murata, T., Uchida, H., Tahara, H., Kaneko, M. K., & Kato, Y. (2018). Detection of Tiger Podoplanin Using the Anti-Cat Podoplanin Monoclonal Antibody PMab-52. Monoclonal antibodies in immunodiagnosis and immunotherapy, 37(5), 224-228. https://doi.org/10.1089/mab.2018.0033