Detection of the PGP9.5 and Tyrosine Hydroxylase mRNAs for Minimal Residual Neuroblastoma Cells in Bone Marrow and Peripheral Blood

Takaaki Yambe Yanagisawa, Yoji Sasahara, Hiromi Fujie, Yoshiyuki Ohashi, Masayoshi Minegishi, Masayuki Itano, Sei Morita, Shigeru Tsuchiya, Yutaka Hayashi, Ryoji Ohi, Tasuke Konno

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

The "touchdown" polymerase chain reaction (PCR) technique has been applied to analyze expression of the neuron-specific protein, PGP9.5, and tyrosine hydroxylase (TH) genes for detection of minimal residual neuroblastoma cells in bone marrow and peripheral blood. PGP9.5 and TH gene products were not detected in any normal samples (n = 72) examined. However, in patients more than 1 year of age with stage III and IV neuroblastoma PGP9.5 mRNA was detected in six of seven bone marrow samples and in tour of eight peripheral blood samples, and TH mRNA in four of seven and three of eight, respectively. The detection sensitivity was up to 10-6 to 10-7 μg of total cellular RNA for PGP9.5 and 10-4 μg for TH. Among forty bone marrow specimens from nineteen patients with neuroblastoma both PGP9.5 and TH mRNAs were detected in six and only PGP9.5 mRNA was detected in ten. Since detection of PGP9.5 and TH gene transcripts by the "touchdown" PCR was highly specific and sensitive, it might be most informative at present to carry out both PGP9.5 and TH mRNA assays for minimal residual neuroblastoma cells in blood and bone marrow.

Original languageEnglish
Pages (from-to)229-240
Number of pages12
JournalTohoku Journal of Experimental Medicine
Volume184
Issue number3
DOIs
Publication statusPublished - 1998 Mar

Keywords

  • Minimal residual cells
  • Neuroblastoma
  • PGP9.5
  • Tyrosine hydroxylase

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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