Detection of surfactant protein-A gene transcript in the cells from pleural effusion for the diagnosis of lung adenocarcinoma

Hiroki Saito, Sanae Shimura, Toshiaki Fushimi, Hiroshi Okayama, Kunio Shirato

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Purpose: To determine whether detecting surfactant protein-A gene transcript in the cells from pleural effusion for the cells from pleural effusion is useful for the diagnosis of lung adenocarcimoma. Patients and methods: We performed reverse transcription polymerase chain reaction (RT- PCR) analysis of SP-A gene transcript in the cells of pleural effusion, including 7 patients with primary lung adenocarcinoma before their treatments. Results: A cDNA segment of SP-A was amplified from the pleural fluid cells of all patients with primary lung adenocarcinoma, indicating the presence of the SP-A gene transcript. None of the remaining patients, including those with metastatic lung adenocarcinoma, showed positive for the SP-A gene transcript. Conclusion: These findings indicate that RT-PCR analysis of the SP-A gene transcript in pleural effusion is useful for the diagnosis of primary lung adenocarcinoma.

Original languageEnglish
Pages (from-to)400-404
Number of pages5
JournalAmerican Journal of Medicine
Volume103
Issue number5
DOIs
Publication statusPublished - 1997 Nov 1

ASJC Scopus subject areas

  • Medicine(all)

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