Detection of spatial localization of Hst-1/Fgf-4 gene expression in brain and testis from adult mice

Hanako Yamamoto, Takahiro Ochiya, Yasushi Takahama, Yasuo Ishii, Noriko Osumi, Hiromi Sakamoto, Masaaki Terada

Research output: Contribution to journalArticlepeer-review

30 Citations (Scopus)

Abstract

HST-1, a member of the fibroblast growth factor (FGF) family (FGF-4), has been shown to be a signaling molecule whose expression is essential for embryonic development. However, HST-1/FGF-4 expression has not been detected or reported in adult tissues so far analysed. To investigate whether there is a possible role of HST-1/FGF-4 in adult stage, we have tarried out a highly sensitive RT-PCR analysis of Hst-1/Fgf-4 gene expression in adult mice tissues. Results show Hst-1/Fgf-4 gene expression in the nervous system, intestines, and testis of normal adult mice. In situ hybridization technique was used to localize Hst-1/Fgf-4 gene expression in the cerebellum and testis from 10-week-old mice. Cell type-specific gene expression was detected: Purkinje cells in the cerebellum and Sertoli cells in testis. These findings suggest that the Hst-1/Fgf-4 gene also plays an important role in adult tissues, and may offer insights into the biological significance of HST-1/FGF-4 in cerebellar and testicular functions.

Original languageEnglish
Pages (from-to)3805-3810
Number of pages6
JournalOncogene
Volume19
Issue number33
DOIs
Publication statusPublished - 2000 Aug 3
Externally publishedYes

Keywords

  • Adult
  • Brain
  • Hst-1/Fgf-4
  • In situ
  • RT-PCR
  • Testis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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