Detection of serum antibody against bovine coccidiosis by using eimeria tenella antigen

Tatsuya Uchida, Hasbullah, Yutaka Nakai, Keiji Ogimoto

Research output: Contribution to journalArticle

Abstract

Antigens of Eimeria tenella sporulated oocysts were applied to detect serum antibody of calf infected with Eimeria. Polypeptides of E. tenella oocyst were strongly proved with the infected bovine serum in immunoblotting. The infected serum also showed higher absorbances than that of normal serum in enzyme linked immunosorbent assay (ELISA) using E. tenella oocyst antigen. Suitable conditions for the ELISA were obtained by using soluble antigen of E. tenella oocysts at 2.5μg/ml and bovine serum at 200 fold dilution.

Original languageEnglish
Pages (from-to)169-171
Number of pages3
JournalJournal of Veterinary Medical Science
Volume57
Issue number1
DOIs
Publication statusPublished - 1995 Jan 1

Keywords

  • ELISA
  • Eimeria tenella antigen
  • bovine coccidiosis

ASJC Scopus subject areas

  • veterinary(all)

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