Detection of serum antibody against bovine coccidiosis by using eimeria tenella antigen

Tatsuya Uchida, Hasbullah, Yutaka Nakai, Keiji Ogimoto

    Research output: Contribution to journalArticlepeer-review

    Abstract

    Antigens of Eimeria tenella sporulated oocysts were applied to detect serum antibody of calf infected with Eimeria. Polypeptides of E. tenella oocyst were strongly proved with the infected bovine serum in immunoblotting. The infected serum also showed higher absorbances than that of normal serum in enzyme linked immunosorbent assay (ELISA) using E. tenella oocyst antigen. Suitable conditions for the ELISA were obtained by using soluble antigen of E. tenella oocysts at 2.5μg/ml and bovine serum at 200 fold dilution.

    Original languageEnglish
    Pages (from-to)169-171
    Number of pages3
    JournalJournal of Veterinary Medical Science
    Volume57
    Issue number1
    DOIs
    Publication statusPublished - 1995

    Keywords

    • ELISA
    • Eimeria tenella antigen
    • bovine coccidiosis

    ASJC Scopus subject areas

    • veterinary(all)

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