Detection of REST expression in the testis using epitope-tag knock-in mice generated by genome editing

Ryuichi Kimura, Yukiko U. Inoue, Takako Kikkawa, Misako Tatehana, Yuki Morimoto, Hitoshi Inada, Shinya Oki, Takayoshi Inoue, Noriko Osumi

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Repressor element 1-silencing transcription factor (REST) is a master regulator that is highly expressed in multipotent stem cells to repress gene networks involving a wide range of biological processes. A recent study has suggested that REST might be involved in a misregulation of its target genes in the embryonic brain of offspring derived from aged fathers. However, detailed analyses of the REST function in spermatogenesis are lacking due to difficulty in the detection of REST protein in specific cell types. Results: To determine localization of REST, we generated an epitope tag knock-in (KI) mouse line with the C-terminus insertion of a podoplanin (PA)-tag at an endogenous Rest locus by the CRISPR/Cas9 system. Localization of the PA-tag was confirmed in neural stem cells marked with Pax6 in the embryonic brain. Moreover, PA-tagged REST was detected in undifferentiated and differentiating spermatogonia as well as Sertoli cells in both neonatal and adult testes. Conclusions: We demonstrate that REST is expressed at the early step of spermatogenesis and suggest a possibility that REST may modulate the epigenetic state of male germline cells. Our KI mice may be useful for studying REST-associated molecular mechanisms of neurodevelopmental and age-related disorders.

Original languageEnglish
JournalDevelopmental Dynamics
DOIs
Publication statusAccepted/In press - 2021

Keywords

  • CRISPR/Cas9
  • knock-in
  • PA-tag
  • REST
  • spermatogenesis

ASJC Scopus subject areas

  • Developmental Biology

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