Detection of pesticide residues using an immunodevice based on negative dielectrophoresis

Javier Ramón-Azcón, Ryouta Kunikata, F. J. Sanchez, M. P. Marco, Hitoshi Shiku, Tomoyuki Yasukawa, Tomokazu Matsue

Research output: Contribution to journalArticlepeer-review

36 Citations (Scopus)


The detection of atrazine using a novel optical immunosensing technique based on negative dielectrophoresis (n-DEP) in microfluidic channels is described. Atrazine is a toxic triazine herbicide within the most frequently used. Polystyrene microparticles (6 μm diameters) modified with bovine serum albumin conjugated with atrazine (atrazine-BSA) were manipulated and captured when subjected to intense n-DEP electric fields. Specifically, particles were trapped when AC voltages with amplitudes of 10 Vpeak and frequencies over 1 MHz were applied to the electrodes. The immunological reaction occurring on the particles for detecting atrazine is based on an indirect competitive assay using a secondary anti-mouse immunogloburin G (IgG) antibody labeled with fluorescein isothiocyanate. The microfluidic device, with three-dimensional microelectrodes, was fabricated comprising two caged areas, allowing two simultaneous measurements inside the same microfluidic channel. The performance of this n-DEP immunosensing technique was evaluated using wine samples. The immunodevice showed a limit of detection for atrazine in buffer samples of 0.11 μg L-1 and in pre-treated wine samples of 6.8 μg L-1; these detection limits are lower than the maximum residue level (MRL) established by the European Community for residues of this herbicide in wine (50 μg L-1). This methodology offers great promise for rapid, simple, cost effective, and on-site analysis of biological, foods and beverages, and environmental samples.

Original languageEnglish
Pages (from-to)1592-1597
Number of pages6
JournalBiosensors and Bioelectronics
Issue number6
Publication statusPublished - 2009 Feb 15


  • Antibodies
  • Atrazine
  • Dielectrophoresis
  • Immunodevice
  • Microfluidic device
  • Microparticles
  • Wine matrix effect

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Biomedical Engineering
  • Electrochemistry

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