Detection of oxidative damages on viral capsid protein for evaluating structural integrity and infectivity of human norovirus

Daisuke Sano, Rosa M. Pintó, Tatsuo Omura, Albert Bosch

Research output: Contribution to journalArticlepeer-review

47 Citations (Scopus)

Abstract

The infectivity evaluation of noncultivatable viruses, such as human norovirus, is crucial to address needs for ensuring the safety in usage of water and marine products. In this work, we tested a new approach to evaluate viral particle integrity, in which oxidatively produced carbonyl groups on viral capsid protein were quantitatively detected. As a result, the decrease in the infectivity of human astrovirus, a representative enteric virus, positively correlated with the amount of oxidative damage on viral particles. Furthermore, when human norovirus was treated by 1 ppm free chlorine for 15 min, 49.93% of virions were recovered as oxidatively damaged particles, which represents a 5-fold increase over those treated by 0.5 ppm free chlorine for 15 min. The detection of the carbonylated viral particles could be a powerful tool for the evaluation of the decrease in the infectivity of noncultivatable viruses.

Original languageEnglish
Pages (from-to)808-812
Number of pages5
JournalEnvironmental Science and Technology
Volume44
Issue number2
DOIs
Publication statusPublished - 2010 Jan 15

ASJC Scopus subject areas

  • Chemistry(all)
  • Environmental Chemistry

Fingerprint Dive into the research topics of 'Detection of oxidative damages on viral capsid protein for evaluating structural integrity and infectivity of human norovirus'. Together they form a unique fingerprint.

Cite this