Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay

Noriko Kimura, Hiroshi Yamamoto, Hiroshi Okamoto, Kunihiko Gotoh, Masahiko Sone, Toraichi Mouri, Kozo Ota, Tokihisa Kimura, Takashi Ohzeki, Yukio Miura

Research output: Contribution to journalArticlepeer-review

6 Citations (Scopus)

Abstract

The production and secretion of multiple peptide hormones and tyrosine hydroxylase by the human neuroblastoma cell line NB-1 and the effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on them were investigated. The presence of messenger RNAs (mRNAs) of vasoactive intestinal peptide (VIP)/peptide histidine methionine (PHM), preprotachykinin, and tyrosine hydroxylase was detectable in the cytoplasm of cultured NB-1 cells by in situ hybridization. Treatment with Bt2cAMP and TPA markedly increased the number of cells immunoreactive to VIP, PHM, neuropeptide Y, Met-enkephalin, substance P and tyrosine hydroxylase and also the contents of VIP and Met-enkephalin in the culture medium. Bt2cAMP and TPA induced morphological changes characteristic of endocrine differentiation, such as an increase in neuroendocrine granules and the development of rough endoplasmic reticulum and Golgi apparatus. The results indicated that treatment with Bt2cAMP and TPA induces the expression of multiple genes of peptide hormone and tyrosine hydroxylase and increases hormone production and secretion through morphological changes into endocrine cells.

Original languageEnglish
Pages (from-to)321-327
Number of pages7
JournalVirchows Archiv B Cell Pathology Including Molecular Pathology
Volume62
Issue number1
DOIs
Publication statusPublished - 1992 Dec 1

Keywords

  • In situ hybridization
  • Met-enkephalin
  • Neuroblastoma
  • Tyrosine hydroxylase
  • Vasoactive intestinal peptide

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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