Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay

Noriko Kimura; Hiroshi Yamamoto; Hiroshi Okamoto; Kunihiko Gotoh; Masahiko Sone; Toraichi Mouri; Kozo Ota; Tokihisa Kimura; Takashi Ohzeki; Yukio Miura

doi:10.1007/BF02899699

Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay

Noriko Kimura, Hiroshi Yamamoto, Hiroshi Okamoto, Kunihiko Gotoh, Masahiko Sone, Toraichi Mouri, Kozo Ota, Tokihisa Kimura, Takashi Ohzeki, Yukio Miura

Surgery

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

The production and secretion of multiple peptide hormones and tyrosine hydroxylase by the human neuroblastoma cell line NB-1 and the effects of dibutyryl cAMP (Bt₂cAMP) and phorbol esters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on them were investigated. The presence of messenger RNA_s (mRNAs) of vasoactive intestinal peptide (VIP)/peptide histidine methionine (PHM), preprotachykinin, and tyrosine hydroxylase was detectable in the cytoplasm of cultured NB-1 cells by in situ hybridization. Treatment with Bt₂cAMP and TPA markedly increased the number of cells immunoreactive to VIP, PHM, neuropeptide Y, Met-enkephalin, substance P and tyrosine hydroxylase and also the contents of VIP and Met-enkephalin in the culture medium. Bt₂cAMP and TPA induced morphological changes characteristic of endocrine differentiation, such as an increase in neuroendocrine granules and the development of rough endoplasmic reticulum and Golgi apparatus. The results indicated that treatment with Bt²cAMP and TPA induces the expression of multiple genes of peptide hormone and tyrosine hydroxylase and increases hormone production and secretion through morphological changes into endocrine cells.

Original language	English
Pages (from-to)	321-327
Number of pages	7
Journal	Virchows Archiv B Cell Pathology Including Molecular Pathology
Volume	62
Issue number	1
DOIs	https://doi.org/10.1007/BF02899699
Publication status	Published - 1992 Dec 1

Keywords

In situ hybridization
Met-enkephalin
Neuroblastoma
Tyrosine hydroxylase
Vasoactive intestinal peptide

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1007/BF02899699

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Kimura, N., Yamamoto, H., Okamoto, H., Gotoh, K., Sone, M., Mouri, T., Ota, K., Kimura, T., Ohzeki, T., & Miura, Y. (1992). Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay. Virchows Archiv B Cell Pathology Including Molecular Pathology, 62(1), 321-327. https://doi.org/10.1007/BF02899699

Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay. / Kimura, Noriko; Yamamoto, Hiroshi; Okamoto, Hiroshi; Gotoh, Kunihiko; Sone, Masahiko; Mouri, Toraichi; Ota, Kozo; Kimura, Tokihisa; Ohzeki, Takashi; Miura, Yukio.

In: Virchows Archiv B Cell Pathology Including Molecular Pathology, Vol. 62, No. 1, 01.12.1992, p. 321-327.

Research output: Contribution to journal › Article › peer-review

Kimura, N, Yamamoto, H, Okamoto, H, Gotoh, K, Sone, M, Mouri, T, Ota, K, Kimura, T, Ohzeki, T & Miura, Y 1992, 'Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay', Virchows Archiv B Cell Pathology Including Molecular Pathology, vol. 62, no. 1, pp. 321-327. https://doi.org/10.1007/BF02899699

Kimura, Noriko ; Yamamoto, Hiroshi ; Okamoto, Hiroshi ; Gotoh, Kunihiko ; Sone, Masahiko ; Mouri, Toraichi ; Ota, Kozo ; Kimura, Tokihisa ; Ohzeki, Takashi ; Miura, Yukio. / Detection of multiple hormones and their mRNAs in human neuroblastoma cell line NB-1 using in situ hybridization, immunocytochemistry and radioimmunoassay. In: Virchows Archiv B Cell Pathology Including Molecular Pathology. 1992 ; Vol. 62, No. 1. pp. 321-327.

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abstract = "The production and secretion of multiple peptide hormones and tyrosine hydroxylase by the human neuroblastoma cell line NB-1 and the effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on them were investigated. The presence of messenger RNAs (mRNAs) of vasoactive intestinal peptide (VIP)/peptide histidine methionine (PHM), preprotachykinin, and tyrosine hydroxylase was detectable in the cytoplasm of cultured NB-1 cells by in situ hybridization. Treatment with Bt2cAMP and TPA markedly increased the number of cells immunoreactive to VIP, PHM, neuropeptide Y, Met-enkephalin, substance P and tyrosine hydroxylase and also the contents of VIP and Met-enkephalin in the culture medium. Bt2cAMP and TPA induced morphological changes characteristic of endocrine differentiation, such as an increase in neuroendocrine granules and the development of rough endoplasmic reticulum and Golgi apparatus. The results indicated that treatment with Bt2cAMP and TPA induces the expression of multiple genes of peptide hormone and tyrosine hydroxylase and increases hormone production and secretion through morphological changes into endocrine cells.",

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AU - Sone, Masahiko

AU - Mouri, Toraichi

AU - Ota, Kozo

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AB - The production and secretion of multiple peptide hormones and tyrosine hydroxylase by the human neuroblastoma cell line NB-1 and the effects of dibutyryl cAMP (Bt2cAMP) and phorbol esters such as 12-O-tetradecanoyl-phorbol-13-acetate (TPA) on them were investigated. The presence of messenger RNAs (mRNAs) of vasoactive intestinal peptide (VIP)/peptide histidine methionine (PHM), preprotachykinin, and tyrosine hydroxylase was detectable in the cytoplasm of cultured NB-1 cells by in situ hybridization. Treatment with Bt2cAMP and TPA markedly increased the number of cells immunoreactive to VIP, PHM, neuropeptide Y, Met-enkephalin, substance P and tyrosine hydroxylase and also the contents of VIP and Met-enkephalin in the culture medium. Bt2cAMP and TPA induced morphological changes characteristic of endocrine differentiation, such as an increase in neuroendocrine granules and the development of rough endoplasmic reticulum and Golgi apparatus. The results indicated that treatment with Bt2cAMP and TPA induces the expression of multiple genes of peptide hormone and tyrosine hydroxylase and increases hormone production and secretion through morphological changes into endocrine cells.

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