Detection of monoclonal proteins by capillary electrophoresis using a zwitterion in the running buffer

Toshihiko Miura, Tadao Funato, Shigemitu Yabuki, Takeshi Sasaki, Mitsuo Kaku

Research output: Contribution to journalArticlepeer-review

11 Citations (Scopus)


Some cases have been reported in which a small monoclonal protein (M-protein) cannot be detected by conventional cellulose acetate membrane electrophoresis (CAE) or capillary zone electrophoresis (CZE) using a short fused-silica capillary. This is probably because these methods do not have the necessary sensitivity or resolution. To overcome this problem, we improved the CZE system by using a longer capillary and adding a zwitterion to the running buffer (pH 10.0). A comparison of CZE and CAE demonstrated that with the exception of α1- and α2-globulin, the correlation was satisfactory in serum samples from 34 patients with M-proteins which had been detected by immunoelectrophoresis. In addition, a comparison of CZE electropherograms with those from CAE showed that small M-proteins that went undetected by CAE could be detected by CZE in four patients whose diseases included epipharyngeal carcinoma, solitary plasmacytoma, Crow-Fukase syndrome and macroglobulinemia. The improved resolution produced by a longer capillary may be effective for the detection of small M-proteins. Copyright (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)87-99
Number of pages13
JournalClinica Chimica Acta
Issue number1-2
Publication statusPublished - 2000 Sep 1


  • Capillary zone electrophoresis
  • Monoclonal protein
  • Zwitterion

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry
  • Biochemistry, medical


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