TY - JOUR
T1 - Detection of monoclonal proteins by capillary electrophoresis using a zwitterion in the running buffer
AU - Miura, Toshihiko
AU - Funato, Tadao
AU - Yabuki, Shigemitu
AU - Sasaki, Takeshi
AU - Kaku, Mitsuo
PY - 2000/9/1
Y1 - 2000/9/1
N2 - Some cases have been reported in which a small monoclonal protein (M-protein) cannot be detected by conventional cellulose acetate membrane electrophoresis (CAE) or capillary zone electrophoresis (CZE) using a short fused-silica capillary. This is probably because these methods do not have the necessary sensitivity or resolution. To overcome this problem, we improved the CZE system by using a longer capillary and adding a zwitterion to the running buffer (pH 10.0). A comparison of CZE and CAE demonstrated that with the exception of α1- and α2-globulin, the correlation was satisfactory in serum samples from 34 patients with M-proteins which had been detected by immunoelectrophoresis. In addition, a comparison of CZE electropherograms with those from CAE showed that small M-proteins that went undetected by CAE could be detected by CZE in four patients whose diseases included epipharyngeal carcinoma, solitary plasmacytoma, Crow-Fukase syndrome and macroglobulinemia. The improved resolution produced by a longer capillary may be effective for the detection of small M-proteins. Copyright (C) 2000 Elsevier Science B.V.
AB - Some cases have been reported in which a small monoclonal protein (M-protein) cannot be detected by conventional cellulose acetate membrane electrophoresis (CAE) or capillary zone electrophoresis (CZE) using a short fused-silica capillary. This is probably because these methods do not have the necessary sensitivity or resolution. To overcome this problem, we improved the CZE system by using a longer capillary and adding a zwitterion to the running buffer (pH 10.0). A comparison of CZE and CAE demonstrated that with the exception of α1- and α2-globulin, the correlation was satisfactory in serum samples from 34 patients with M-proteins which had been detected by immunoelectrophoresis. In addition, a comparison of CZE electropherograms with those from CAE showed that small M-proteins that went undetected by CAE could be detected by CZE in four patients whose diseases included epipharyngeal carcinoma, solitary plasmacytoma, Crow-Fukase syndrome and macroglobulinemia. The improved resolution produced by a longer capillary may be effective for the detection of small M-proteins. Copyright (C) 2000 Elsevier Science B.V.
KW - Capillary zone electrophoresis
KW - Monoclonal protein
KW - Zwitterion
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U2 - 10.1016/S0009-8981(00)00277-1
DO - 10.1016/S0009-8981(00)00277-1
M3 - Article
C2 - 10900295
AN - SCOPUS:0033934605
VL - 299
SP - 87
EP - 99
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
SN - 0009-8981
IS - 1-2
ER -