Detection of microspotted carcinoembryonic antigen on a glass substrate by scanning electrochemical microscopy

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Abstract

Microspots of carbinoembryonic antigen (CEA) on glass substrates were characterized by scanning electrochemical microscopy (SECM). CEA was immobilized via a sandiwch method using horseradish peroxidase (HRP)-labeled anti-CEA. The reduction current of the oxidized form of ferrocenylmethanol generated by the HRP reaction was monitored to view SECM images. This method detects as low as ∼104 CEA molecules in a single 20-μm-radius spot.

Original languageEnglish
Pages (from-to)1276-1278
Number of pages3
JournalAnalytical Chemistry
Volume68
Issue number7
DOIs
Publication statusPublished - 1996 Jan 1

ASJC Scopus subject areas

  • Analytical Chemistry

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