Detection of microsatellite instability in cancers by arbitrarily primed-PCR fingerprinting using a fluorescently labeled primer (FAP-PCR)

Jun Yasuda, Hidefumi Kashiwabara, Keita Kawakami, Kazutsugu Uematsu, Kokichi Sugano, Manuel Perucho, Takao Sekiya

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The microsatellite mutator phenotype (MMP), detected as a change in the number of repeating units in hundreds of thousands of microsatellite sequences in the tumor cell genome, underlies the carcinogenesis of a variety of tumors including sporadic and hereditary nonpolyposis colon cancers. This enhanced microsatellite instability was discovered using arbitrarily primed polymerase chain reaction (AP-PCR) fingerprinting of DNA from colon cancers. In this study, we found an arbitrary primer that can amplify multiple DNA fragments containing repeated sequences, including the poly A tracts found in the Alu repeats of the human genome. The combined use of primer labeling with fluorescence and an automated DNA sequencing analysis of AP-PCR products (FAP-PCR) detected alterations in fingerprint bands in all DNA samples previously determined to belong to the MMP. Fluorescent AP-PCR fingerprinting using this single arbitrary primer provides a convenient and efficient method for detecting tumor specific fingerprint alterations that are usually undetectable by conventional fingerprinting.

Original languageEnglish
Pages (from-to)563-570
Number of pages8
JournalBiological Chemistry
Volume377
Issue number9
DOIs
Publication statusPublished - 1996 Sep

Keywords

  • FAP-PCR fingerprinting
  • Microsatellite mutator phenotype
  • Mutator mutations
  • PCR with a fluorescently labeled primer

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Clinical Biochemistry

Fingerprint Dive into the research topics of 'Detection of microsatellite instability in cancers by arbitrarily primed-PCR fingerprinting using a fluorescently labeled primer (FAP-PCR)'. Together they form a unique fingerprint.

Cite this