Detection of Lion Podoplanin Using the Antitiger Podoplanin Monoclonal Antibody PMab-231

Yukinari Kato, Junko Takei, Masato Sano, Teizo Asano, Yusuke Sayama, Kazuyuki Uchida, Takayuki Nakagawa, Mika K. Kaneko

Research output: Contribution to journalArticlepeer-review

Abstract

Monoclonal antibodies (mAbs) that specifically target podoplanin (PDPN), a marker for type I alveolar cells, are needed for immunohistochemical analyses. Anti-PDPN mAbs are available for many species, including human, mouse, rat, rabbit, dog, cat, bovine, pig, Tasmanian devil, alpaca, tiger, whale, goat, horse, bear, and sheep PDPNs. However, no antilion PDPN (lioPDPN) antibody has been developed. In this study, possible cross-reaction between available anti-PDPN mAbs and lioPDPN was examined. Immunohistochemical analysis showed that antitiger PDPN mAb PMab-231 (IgG2a, kappa) reacted with type I alveolar cells from lion lung, indicating that PMab-231 is useful for the detection of lioPDPN.

Original languageEnglish
Pages (from-to)91-94
Number of pages4
JournalMonoclonal antibodies in immunodiagnosis and immunotherapy
Volume39
Issue number3
DOIs
Publication statusPublished - 2020 Jun

Keywords

  • PDPN
  • PMab-231
  • lioPDPN
  • lion podoplanin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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