Detection of Legionella species by polymerase chain reaction

M. Iwamoto, H. Koga, S. Kohno, Mitsuo Kaku, K. Hara

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

The nested polymerase chain reaction (PCR) was clinically investigated to detect the mip gene of Legionella species. PCR detected 20 clinically important Legionella species, such as L. pneumophila, L. micdadei, and L. bozemanii. Eight species of Gram-positive and -negative bacteria other than Legionella species were negative for PCR. The sensitivity of PCR determined by the detection limit of DNA quantity was 1.0 pg for the first PCR, but the sensitivity increased approximately 100-fold to 10 fg following the second PCR. The nested PCR was applied to detect L. pneumophila in the sputum and pleural effusion fluid obtained from a patient with Legionnaires' disease. Both fluids were PCR positive, but culture was negative for L. pneumophila. Our results indicated that the nested PCR may be a useful tool for the rapid detection of L. pneumophila and the clinical diagnosis of Legionnaires' disease.

Original languageEnglish
Pages (from-to)99-103
Number of pages5
JournalSerodiagnosis and Immunotherapy in Infectious Disease
Volume7
Issue number3
DOIs
Publication statusPublished - 1995 Jan 1

Keywords

  • Legionella
  • polymerase chain reaction
  • rapid diagnosis

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)

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