Detection of immunoreactive endothelin in plasma of hemodialysis patients

Kazuhito Totsune, Toraichi Mouri, Kazuhiro Takahashi, Minoru Ohneda, Masahiko Sone, Takao Saito, Kaoru Yoshinaga

Research output: Contribution to journalArticlepeer-review

56 Citations (Scopus)

Abstract

Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C18 cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C18 cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalFEBS Letters
Volume249
Issue number2
DOIs
Publication statusPublished - 1989 Jun 5

Keywords

  • (Human plasma)
  • Endothelin
  • Hemodialysis
  • Immunoreactivity
  • Radioimmunoassay

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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