Detection of immunoreactive endothelin in plasma of hemodialysis patients

Kazuhito Totsune; Toraichi Mouri; Kazuhiro Takahashi; Minoru Ohneda; Masahiko Sone; Takao Saito; Kaoru Yoshinaga

doi:10.1016/0014-5793(89)80631-3

Detection of immunoreactive endothelin in plasma of hemodialysis patients

Kazuhito Totsune, Toraichi Mouri, Kazuhiro Takahashi, Minoru Ohneda, Masahiko Sone, Takao Saito, Kaoru Yoshinaga

Research output: Contribution to journal › Article › peer-review

51 Citations (Scopus)

Abstract

Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C₁₈ cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C₁₈ cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.

Original language	English
Pages (from-to)	239-242
Number of pages	4
Journal	FEBS Letters
Volume	249
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(89)80631-3
Publication status	Published - 1989 Jun 5

Keywords

(Human plasma)
Endothelin
Hemodialysis
Immunoreactivity
Radioimmunoassay

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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title = "Detection of immunoreactive endothelin in plasma of hemodialysis patients",

abstract = "Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C18 cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C18 cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.",

keywords = "(Human plasma), Endothelin, Hemodialysis, Immunoreactivity, Radioimmunoassay",

author = "Kazuhito Totsune and Toraichi Mouri and Kazuhiro Takahashi and Minoru Ohneda and Masahiko Sone and Takao Saito and Kaoru Yoshinaga",

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T1 - Detection of immunoreactive endothelin in plasma of hemodialysis patients

AU - Totsune, Kazuhito

AU - Mouri, Toraichi

AU - Takahashi, Kazuhiro

AU - Ohneda, Minoru

AU - Sone, Masahiko

AU - Saito, Takao

AU - Yoshinaga, Kaoru

PY - 1989/6/5

Y1 - 1989/6/5

N2 - Two types of radioimmunoassay (RIA) methods for measuring endothelin (ET) in human plasma were developed. One was an extraction procedure using a Sep-Pak C18 cartridge, the other being a direct method. By the extraction method, plasma ET levels were lower than the detectable limit (7 pg/ml) in normal subjects and elevated in hemodialysis patients. The absolute values obtained via the direct method were 20-times higher than those from extraction. Gel-filtration experiments revealed that this discrepancy was mainly due to immunoreactive (IR-) endothelin-like substances of high molecular mass near 11.6 kDa (large IR-ET). Extraction of the peptide by the C18 cartridge could eliminate interference by large IR-ET and is important in the accurate measurement of ET concentrations in plasma.

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KW - (Human plasma)

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KW - Hemodialysis

KW - Immunoreactivity

KW - Radioimmunoassay

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