Detection of Human Papillomavirus Type 52B in Cervical Dysplastic Tissue

Nobuo Yaegashi, Ryo Konno, Kazuo Shikano, Shinji Sato, Akira Yajima, Hajime Yajima

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Yaegashi, N., Yajima, H., Konno, R., Shikano, K., Sato, S. and Yajima, A. Detection of Human Papillomavirus Type 52b in Cervical Dysplastic Tissue. Tohoku J. Exp. Med., 1989, 159 (3), 215-219-We examined infection in the tissue of cervical dysplasia of human papillomavirus (HPV) 52b, which we recently isolated from cervical cancer. To screen a lot of samples, we used an assay system with an HPV DNA typing kit containing an HPV 33 probe that cross-hybridizes with HPV 52b DNA. DNA samples from 123 patients with cervical dysplasia were first screened with this kit. Sixteen of these appeared to be positive, and they were subjected to Southern blot hybridization with the HPV 52b probe under highly stringent conditions. Of the 16 samples, two were found to have HPV 52b genome DNA. Our results, together with the fact that HPV 52b was isolated from cervical cancer, seem to support the notion that HPV 52b infection is closely associated with the onset of cervical cancer.

Original languageEnglish
Pages (from-to)215-219
Number of pages5
Journalthe tohoku journal of experimental medicine
Volume159
Issue number3
DOIs
Publication statusPublished - 1989 Jan 1

Keywords

  • 52b
  • cervical dysplasia
  • human papillomavirus
  • southern blotting

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Detection of Human Papillomavirus Type 52B in Cervical Dysplastic Tissue'. Together they form a unique fingerprint.

  • Cite this