Detection of Helicobacter pylori in cow's milk

S. Fujimura, T. Kawamura, S. Kato, H. Tateno, A. Watanabe

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    53 Citations (Scopus)

    Abstract

    Aims: To investigate the existence of Helicobacter pylori in cow's milk as one of the foods which most Japanese children eat. Methods and Results: Detection of H. pylori was demonstrated by the semi-nested polymerase chain reaction (PCR), a culture method and electron microscopy. Semi-nested PCR demonstrated the ureA gene of H. pylori in 13 of 18 (72.2%) raw milk samples and in 11 of 20 (55%) commercial pasteurized milk samples. Helicobacter pylori binding immunomagnetic beads with H. pylori-specific goat anti-H. pylori antibody was shown by electron microscopy in both raw and pasteurized milk positive for the ureA gene. Helicobacter pylori was cultured in one raw milk sample, whereas it was not cultured in pasteurized milk samples. Conclusions, Significance and Impact of the Study: There is a possibility that cow's milk is a transmission vehicle in childhood H. pylori infection, although we failed to confirm the survival of H. pylori in pasteurized milk.

    Original languageEnglish
    Pages (from-to)504-507
    Number of pages4
    JournalLetters in Applied Microbiology
    Volume35
    Issue number6
    DOIs
    Publication statusPublished - 2002 Dec 1

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology

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