Detection of Glutamate in Optically Trapped Single Nerve Terminals by Raman Spectroscopy

Katsuhiro Ajito, Chunxi Han, Keiichi Torimitsu

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

Glutamate is the major excitatory neurotransmitter and is of particular interest in light of current models of memory and learning. The paper describes the first in situ detection of glutamate in single nerve terminals (synaptosomes), which is achieved by using laser trapping Raman spectroscopy. The near-infrared laser light captures a single synaptosome obtained from a Wister rat brain. The release of glutamate in a single laser-trapped synaptosome was detected by subtracting the Raman spectrum before depolarization from that after depolarization with the addition of the K +-channel blocker, 4-aminopyridine. The result indicated that the single synaptosome released ∼3 amol of glutamate and that the release rate depended on the 4-aminopyridine concentration.

Original languageEnglish
Pages (from-to)2506-2510
Number of pages5
JournalAnalytical Chemistry
Volume76
Issue number9
DOIs
Publication statusPublished - 2004 May 1
Externally publishedYes

ASJC Scopus subject areas

  • Analytical Chemistry

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