Abstract
Background/Aim: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for non-small cell lung cancer. EGFR gene mutations at codons 746-753 promote constitutive EGFR activation and result in worst prognosis. However, these mutations augment the therapeutic effect of EGFR-tyrosine kinase inhibitor. Therefore, the detection of EGFR gene mutations is important for determining treatment planning. The aim of the study was to establish a method to detect EGFR gene mutations at codons 746-753. Materials and Methods: EGFR gene mutation at codons 746-753 in six cancer cell lines were investigated. A loop-mediated isothermal amplification (LAMP)-based procedure was developed, that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented LAMP can amplify the DNA fragment of the EGFR gene with codons 746-753 mutations within 30 min. Moreover, boiled cells can work as template resources. Conclusion: Mutation oriented-LAMP assay for EGFR gene mutation is sensitive on extracted DNA. This procedure would be capable of detecting EGFR gene mutation in sputum, pleural effusion, broncho-alveolar lavage fluid or trans-bronchial lung biopsy by chair side.
Original language | English |
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Pages (from-to) | 2093-2099 |
Number of pages | 7 |
Journal | Anticancer research |
Volume | 38 |
Issue number | 4 |
DOIs | |
Publication status | Published - 2018 Apr |
Externally published | Yes |
Keywords
- EGFR mutation
- LAMP
- Molecular targeted therapy
- Stratification biomarker.
ASJC Scopus subject areas
- Oncology
- Cancer Research