Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy

T. Miyoshi, R. Yamaguchi, K. Ishibashi, K. Miyamoto, Yasuo Kimura, M. Niwano

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound biomolecular species through IR spectral profiles of biomolecules of interest, allowing us to detect biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transformation of single-stranded DNA molecules (oligonucleotides) in the micro-channels.

Original languageEnglish
Title of host publicationProceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages525-528
Number of pages4
ISBN (Print)1424409519, 9781424409518
DOIs
Publication statusPublished - 2007
Event20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan
Duration: 2007 Jan 212007 Jan 25

Publication series

NameProceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)1084-6999

Other

Other20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
CountryJapan
CityKobe
Period07/1/2107/1/25

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

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