TY - GEN
T1 - Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy
AU - Miyoshi, T.
AU - Yamaguchi, R.
AU - Ishibashi, K.
AU - Miyamoto, K.
AU - Kimura, Yasuo
AU - Niwano, M.
PY - 2007
Y1 - 2007
N2 - Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound biomolecular species through IR spectral profiles of biomolecules of interest, allowing us to detect biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transformation of single-stranded DNA molecules (oligonucleotides) in the micro-channels.
AB - Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound biomolecular species through IR spectral profiles of biomolecules of interest, allowing us to detect biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transformation of single-stranded DNA molecules (oligonucleotides) in the micro-channels.
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U2 - 10.1109/memsys.2007.4433120
DO - 10.1109/memsys.2007.4433120
M3 - Conference contribution
AN - SCOPUS:52249099878
SN - 1424409519
SN - 9781424409518
T3 - Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
SP - 525
EP - 528
BT - Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Y2 - 21 January 2007 through 25 January 2007
ER -