Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy

T. Miyoshi; R. Yamaguchi; K. Ishibashi; K. Miyamoto; Yasuo Kimura; M. Niwano

doi:10.1109/memsys.2007.4433120

Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy

T. Miyoshi, R. Yamaguchi, K. Ishibashi, K. Miyamoto, Yasuo Kimura, M. Niwano

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound biomolecular species through IR spectral profiles of biomolecules of interest, allowing us to detect biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transformation of single-stranded DNA molecules (oligonucleotides) in the micro-channels.

Original language	English
Title of host publication	Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007
Publisher	Institute of Electrical and Electronics Engineers Inc.
Pages	525-528
Number of pages	4
ISBN (Print)	1424409519, 9781424409518
DOIs	https://doi.org/10.1109/memsys.2007.4433120
Publication status	Published - 2007
Event	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007 - Kobe, Japan Duration: 2007 Jan 21 → 2007 Jan 25

Publication series

Name	Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)
ISSN (Print)	1084-6999

Other

Other	20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007
Country	Japan
City	Kobe
Period	07/1/21 → 07/1/25

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Mechanical Engineering
Electrical and Electronic Engineering

Access to Document

10.1109/memsys.2007.4433120

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Miyoshi, T., Yamaguchi, R., Ishibashi, K., Miyamoto, K., Kimura, Y., & Niwano, M. (2007). Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007 (pp. 525-528). [4433120] (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/memsys.2007.4433120

Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy. / Miyoshi, T.; Yamaguchi, R.; Ishibashi, K.; Miyamoto, K.; Kimura, Yasuo; Niwano, M.

Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. Institute of Electrical and Electronics Engineers Inc., 2007. p. 525-528 4433120 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Miyoshi, T, Yamaguchi, R, Ishibashi, K, Miyamoto, K, Kimura, Y & Niwano, M 2007, Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy. in Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007., 4433120, Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Institute of Electrical and Electronics Engineers Inc., pp. 525-528, 20th IEEE International Conference on Micro Electro Mechanical Systems, MEMS 2007, Kobe, Japan, 07/1/21. https://doi.org/10.1109/memsys.2007.4433120

Miyoshi T, Yamaguchi R, Ishibashi K, Miyamoto K, Kimura Y, Niwano M. Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy. In Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. Institute of Electrical and Electronics Engineers Inc. 2007. p. 525-528. 4433120. (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)). https://doi.org/10.1109/memsys.2007.4433120

Miyoshi, T. ; Yamaguchi, R. ; Ishibashi, K. ; Miyamoto, K. ; Kimura, Yasuo ; Niwano, M. / Detection of DNA molecules in micro fluidic channels by infrared absorption spectroscopy. Proceedings - CIS Workshops 2007, 2007 International Conference on Computational Intelligence and Security Workshops, CISW 2007. Institute of Electrical and Electronics Engineers Inc., 2007. pp. 525-528 (Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS)).

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abstract = "Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound biomolecular species through IR spectral profiles of biomolecules of interest, allowing us to detect biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transformation of single-stranded DNA molecules (oligonucleotides) in the micro-channels.",

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AB - Infrared absorption spectroscopy (IRAS) in a multiple internal reflection (MIR) geometry is integrated with silicon-based micro-fluidic channels to allow detection and separation of DNA molecules in the micro-channels. The micro-channels were fabricated on a Si MIR-prism using the conventional silicon lithographic techniques. In the present method we can probe both solution-phase and surface-bound biomolecular species through IR spectral profiles of biomolecules of interest, allowing us to detect biomolecules without fluorescence labeling. The applicability of this approach is demonstrated with a representative test case: transformation of single-stranded DNA molecules (oligonucleotides) in the micro-channels.

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