TY - JOUR
T1 - Detection of DNA abnormalities by arbitrarily primed PCR fingerprinting
T2 - Amplification of the MDM2 gene in a mediastinum fibrosarcoma
AU - Kuchiki, Hideo
AU - Yasuda, Jun
AU - Kayama, Takamasa
AU - Murakami, Yoshinori
AU - Sekiya, Takao
N1 - Funding Information:
This work was supported in part by Grants-in-Aid from the Ministry of Health and Welfare for the 2nd Term Comprehensive 10- Year Strategy for Cancer Control, Japan, a Grant for Research on Aging and Health from the Ministry of Health and Welfare, and a Research Grant on Human Genome and Gene Therapy from the Ministry of Health and Welfare, and a Grant-in-Aid from the Ministry of Education, Science, Sports and Culture of Japan. H.K. is a recipient of a Research Resident Fellowship from the Foundation for Promotion of Cancer Research of Japan.
PY - 1999/5/10
Y1 - 1999/5/10
N2 - Arbitrarily primed PCR (AP-PCR) fingerprinting method is easy and useful for analysis of genetic alterations in anonymous chromosomal regions. We applied this technology to analysis of DNA from human primary cancers and found amplification of a DNA fragment in a mediastinum fibrosarcoma. PCR-based analysis of radiation hybrid panels following cloning and nucleotide sequence determination of the fragment revealed that it was derived from a region of chromosome 12q13-q15. In this region, the MDM2 and IFNG genes were noted as known genes that could be involved in human carcinogenesis. Southern blot analysis of genomic DNA of the tumor revealed the amplification of the MDM2 gene together with the fragment locus, but not the IFNG gene. Our results demonstrated that detection of DNA alterations by AP-PCR fingerprinting without any previous knowledge of the genes and subsequent analysis of radiation hybrid panels could lead to easy identification of candidates for genes involved in carcinogenesis.
AB - Arbitrarily primed PCR (AP-PCR) fingerprinting method is easy and useful for analysis of genetic alterations in anonymous chromosomal regions. We applied this technology to analysis of DNA from human primary cancers and found amplification of a DNA fragment in a mediastinum fibrosarcoma. PCR-based analysis of radiation hybrid panels following cloning and nucleotide sequence determination of the fragment revealed that it was derived from a region of chromosome 12q13-q15. In this region, the MDM2 and IFNG genes were noted as known genes that could be involved in human carcinogenesis. Southern blot analysis of genomic DNA of the tumor revealed the amplification of the MDM2 gene together with the fragment locus, but not the IFNG gene. Our results demonstrated that detection of DNA alterations by AP-PCR fingerprinting without any previous knowledge of the genes and subsequent analysis of radiation hybrid panels could lead to easy identification of candidates for genes involved in carcinogenesis.
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U2 - 10.1006/bbrc.1999.0636
DO - 10.1006/bbrc.1999.0636
M3 - Article
C2 - 10329377
AN - SCOPUS:0033542137
VL - 258
SP - 271
EP - 277
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 2
ER -