Detection of diatoms in blood by a combination of membrane filtering and chemical digestion

Masato Funayama, Yasuhiro Aoki, Ismail M. Sebetan, Kaoru Sagisaka

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

An improved method for detecting diatom in blood is reported. Blood of cadavers was obtained by cardiac puncture at inquest or from the left atrium directly at autopsy. The blood was hemolyzed by sodium dodecyl sulfate (SDS) and filtered through membrane filter (47 mm in diameter and 5 μm in pore size). When the blood was putrefied, two or three pieces of membranes filters were needed because of choking membrane pore. The membrane filters were digested with fuming nitric acid and diluted with distilled water. The diluted solution was filtered through membrane filter (25 mm in diameter) again. After drying the membrane filter was immersed in oil. Diatoms on the membrane filter were clearly observed microscopically.

Original languageEnglish
Pages (from-to)175-182
Number of pages8
JournalForensic Science International
Volume34
Issue number3
DOIs
Publication statusPublished - 1987 Jul

Keywords

  • Diatom test
  • Drowning
  • Membrane filtering

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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