Detection of clone-specific immunoglobulin heavy chain genes in the bone marrow of B-cell-lineage lymphoma after treatment

Atsushi Hoshino, Tadao Funato, Yasuhiko Munakata, Tomonori Ishii, Shori Abe, Kenichi Ishizawa, Ryo Ichinohasama, Junichi Kameoka, Kuniaki Meguro, Takeshi Sasaki

Research output: Contribution to journalArticlepeer-review

3 Citations (Scopus)

Abstract

In order to determine the appropriate treatment of malignant lymphoma, it is important to know the degree to which extra-nodal invasion of lymphoma cells has occurred. We amplified complementarity-determining region (CDR) III genes in 64% of lymph node samples at the onset or relapse of B-cell-lineage non-Hodgkin's lymphoma (NHL) in 22 patients. By using a clone-specific CDR III probe in each patient, we were able to detect minimal residual disease (MRD) of lymphoma cells in the bone marrow and/or blood in 9 out of 14 cases (64.2%) at the onset of the disease or relapse, whereas abnormal cells in the bone marrow and/or blood were identified by routine morphological analysis in only 4 out of 22 cases (18.2%). This indicates that extranodal invasion of malignant cells may be common in patients with NHL. In some cases, the clone-specific CDR III gene was still expressed in the samples of bone marrow and/or peripheral blood even after chemotherapy, when other markers associated with NHL were no longer expressed. Five out of six cases in this group had a worse outcome associated with NHL. On the other hand, most of the cases whose clone-specific CDR III gene was no longer expressed in the bone marrow and/or in circulation after treatment had a relatively fair prognosis. These results indicate that the detection at molecular level of MRD in extranodal organs may prove useful as a predictor of prognosis for NHL.

Original languageEnglish
Pages (from-to)155-164
Number of pages10
JournalTohoku Journal of Experimental Medicine
Volume203
Issue number3
DOIs
Publication statusPublished - 2004 Jul

Keywords

  • Complementarity-determining region (CDR) III genes
  • Immunoglobulin heavy chain (IgH)
  • Minimal residual disease (MRD)
  • Polymerase chain reaction (PCR)
  • non-Hodgkin's lymphoma (NHL)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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