Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor

Takumi Higashiyama, Akihiro Katsuyama, Hideki Otori, Toru Kamimura, Atsushi Uehara, Miho Kainuma, Ryo Takumi, Yukako Kudo, Masayuki Ebina, Katsumi Mochitate, Tasuku Kon, Yasubumi Furuya, Hideaki Kikuchi

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The rat lung epithelial cell line SV40-T2 was used to develop a cellular biosensing system to assay for environmental toxicants. The novel approach on which this system is based involves direct attachment of cultured rat or human cells onto a cell-adhesive matrix on the device through which shear horizontal surface acoustic waves (SH-SAW) are transmitted using 50 MHz SAW resonator. This novel design enables sensitive monitoring of changes of the electrophysical characteristics of cells, such as their conductivity and relative permittivity. A time-dependent change of phase of SAW and change of insertion loss (change of amplitude) were observed when the cells were treated with 0.5 or 1.0 mM H 2O2. The change of insertion loss was biphasic, with an early phase (1-3 h) and a late phase (3-6 h). The late phase coincided with the destruction of cell-cell tight junctions detected by measurement of the transepithelial electrical resistance and paracellular permeability; in contrast, the early phase coincided with the destruction of intracellular actin filaments by H2O2. The early-phase effect of H 2O2 on phase shift may be attributable to the change of intracellular permittivity by a change of cellular polarity. Immunofluorescence microscopy showed the disappearance of zonula occludens protein 1 from the region of cell-cell contact. These results suggest the correlation between the change of insertion loss as an SAW parameter and the destruction of tight junctions of the cells on the SH-SAW device in the late phase.

Original languageEnglish
Pages (from-to)1430-1438
Number of pages9
JournalUltrasonics
Volume54
Issue number6
DOIs
Publication statusPublished - 2014 Aug
Externally publishedYes

Keywords

  • Epithelial cell
  • Hydrogen peroxide
  • Shear horizontal surface acoustic wave
  • Tight junction
  • Transepithelial electrical resistance

ASJC Scopus subject areas

  • Acoustics and Ultrasonics

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  • Cite this

    Higashiyama, T., Katsuyama, A., Otori, H., Kamimura, T., Uehara, A., Kainuma, M., Takumi, R., Kudo, Y., Ebina, M., Mochitate, K., Kon, T., Furuya, Y., & Kikuchi, H. (2014). Detection of cellular damage by hydrogen peroxide using SV40-T2 cells on shear horizontal surface acoustic wave (SH-SAW) sensor. Ultrasonics, 54(6), 1430-1438. https://doi.org/10.1016/j.ultras.2014.04.026