Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase

M. Miyata; A. Kogure; H. Sato; E. Kodama; H. Watanabe; H. Ohira; M. Kuroda; T. Takagi; Y. Sato; R. Kasukawa

doi:10.1007/BF02207935

Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase

M. Miyata, A. Kogure, H. Sato, E. Kodama, H. Watanabe, H. Ohira, M. Kuroda, T. Takagi, Y. Sato, R. Kasukawa

Disaster Medical Science Division

Research output: Contribution to journal › Article › peer-review

14 Citations (Scopus)

Abstract

The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of mycobacterial HSP65.

Original language	English
Pages (from-to)	673-677
Number of pages	5
Journal	Clinical Rheumatology
Volume	14
Issue number	6
DOIs	https://doi.org/10.1007/BF02207935
Publication status	Published - 1995 Nov

Keywords

Autoimmune Hepatitis
Human 60 KD Heat Shock Protein
Molecular Mimicry
Mycobacterial 65 KD Heat Shock Protein
Superoxide Dismutase

ASJC Scopus subject areas

Rheumatology

Access to Document

10.1007/BF02207935

Fingerprint Dive into the research topics of 'Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase'. Together they form a unique fingerprint.

Cite this

Miyata, M., Kogure, A., Sato, H., Kodama, E., Watanabe, H., Ohira, H., Kuroda, M., Takagi, T., Sato, Y., & Kasukawa, R. (1995). Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase. Clinical Rheumatology, 14(6), 673-677. https://doi.org/10.1007/BF02207935

Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase. / Miyata, M.; Kogure, A.; Sato, H.; Kodama, E.; Watanabe, H.; Ohira, H.; Kuroda, M.; Takagi, T.; Sato, Y.; Kasukawa, R.

In: Clinical Rheumatology, Vol. 14, No. 6, 11.1995, p. 673-677.

Research output: Contribution to journal › Article › peer-review

Miyata, M, Kogure, A, Sato, H, Kodama, E, Watanabe, H, Ohira, H, Kuroda, M, Takagi, T, Sato, Y & Kasukawa, R 1995, 'Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase', Clinical Rheumatology, vol. 14, no. 6, pp. 673-677. https://doi.org/10.1007/BF02207935

Miyata, M. ; Kogure, A. ; Sato, H. ; Kodama, E. ; Watanabe, H. ; Ohira, H. ; Kuroda, M. ; Takagi, T. ; Sato, Y. ; Kasukawa, R. / Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase. In: Clinical Rheumatology. 1995 ; Vol. 14, No. 6. pp. 673-677.

@article{1ebe61bab9584909b79842a3a7d5e695,

title = "Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase",

abstract = "The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of mycobacterial HSP65.",

keywords = "Autoimmune Hepatitis, Human 60 KD Heat Shock Protein, Molecular Mimicry, Mycobacterial 65 KD Heat Shock Protein, Superoxide Dismutase",

author = "M. Miyata and A. Kogure and H. Sato and E. Kodama and H. Watanabe and H. Ohira and M. Kuroda and T. Takagi and Y. Sato and R. Kasukawa",

year = "1995",

month = nov,

doi = "10.1007/BF02207935",

language = "English",

volume = "14",

pages = "673--677",

journal = "Clinical Rheumatology",

issn = "0770-3198",

publisher = "Springer London",

number = "6",

}

TY - JOUR

T1 - Detection of antibodies to 65 KD heat shock protein and to human superoxide dismutase in autoimmune hepatitis-molecular mimicry between 65 KD heat shock protein and superoxide dismutase

AU - Miyata, M.

AU - Kogure, A.

AU - Sato, H.

AU - Kodama, E.

AU - Watanabe, H.

AU - Ohira, H.

AU - Kuroda, M.

AU - Takagi, T.

AU - Sato, Y.

AU - Kasukawa, R.

PY - 1995/11

Y1 - 1995/11

N2 - The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of mycobacterial HSP65.

AB - The antibody to 65 KD mycobacterial heat shock protein (HSP65) and antibody to human superoxide dismutase (H-SOD) were measured by ELISA in patients with autoimmune hepatitis (AIH), and results were compared with those of patients with chronic active hepatitis C (CAH-C) or systemic lupus erythematosus (SLE) and normal subjects (NS). Patients with AIH had significantly higher OD values of anti-HSP65 antibody and anti-H-SOD antibody compared with those of patients with CAH-C or SLE and NS. OD values of anti-HSP65 antibody were correlated with those of anti-SOD antibody. Affinity-purified anti-SOD antibody reacted with HSP65. Analysis of the amino acid sequence of human SOD showed that 7 segments, corresponding to r to 25 amino acid residues, exhibited 50 to 71% homology with that of mycobacterial HSP65.

KW - Autoimmune Hepatitis

KW - Human 60 KD Heat Shock Protein

KW - Molecular Mimicry

KW - Mycobacterial 65 KD Heat Shock Protein

KW - Superoxide Dismutase

UR - http://www.scopus.com/inward/record.url?scp=0028884934&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028884934&partnerID=8YFLogxK

U2 - 10.1007/BF02207935

DO - 10.1007/BF02207935

M3 - Article

C2 - 8608687

AN - SCOPUS:0028884934

VL - 14

SP - 673

EP - 677

JO - Clinical Rheumatology

JF - Clinical Rheumatology

SN - 0770-3198

IS - 6

ER -