Detection of anammox activity and 16S rRNA genes in ravine paddy field soil

Yoshinori Sato, Hiroyuki Ohta, Takao Yamagishi, Yong Guo, Tomoyasu Nishizawa, M. Habibur Rahman, Hisao Kuroda, Task Kato, Masanori Saito, Ikuo Yoshinaga, Kazuyuki Inubushi, Yuichi Suwa

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

An anammox assay involving a 15N tracer and gas chromatography-mass spectrometry revealed that the potential anammox activity accounted for 1 to 5% of total N2 production in a ravine paddy field, Japan. Among four 4-cm-deep layers, the top layer showed the highest activity. Clone libraries showed that the DNA in the top layer contained sequences related to those of Candidatus 'Brocadia fulgida', Ca. 'B. anammoxidans', and Ca. 'Kuenenia stuttgartiensis'. These results suggest that a specific population of anammox bacteria was present in paddy soils, although a small part of dinitrogen gas was emitted from the soil via anammox.

Original languageEnglish
Pages (from-to)316-319
Number of pages4
JournalMicrobes and environments
Volume27
Issue number3
DOIs
Publication statusPublished - 2012

Keywords

  • Anaerobic ammonium oxidation
  • Nitrogen removal
  • Ravine paddy field soil

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Soil Science
  • Plant Science

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