Detection of 210 kDa receptor protein for a leaf-movement factor by using novel photoaffinity probes

Tomohiko Fujii, Yoshiyuki Manabe, Takanori Sugimoto, Minoru Ueda

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Circadian rhythmic plant leaf-movement, called nyctinasty, is controlled by a time-course change in the internal concentration of the leaf-movement factor in the plant body. We revealed that specific binding proteins (210 and 180 kDa) for the leaf-movement factor, potassium lespedezate (1), are contained in the plasma membrane of the plant motor cell by using novel synthetic photoaffinity probes. These proteins are localized on the motor cell in the plant body, and would be potential receptors for the leaf-movement factor to control the leaf-movement. Our study is a rare successful result of the detection of membrane receptors by using a synthetic photoaffinity probe designed on a biologically active natural product. And these results also advance a guideline for probe design towards successful photoaffinity labeling.

Original languageEnglish
Pages (from-to)7874-7893
Number of pages20
JournalTetrahedron
Volume61
Issue number33
DOIs
Publication statusPublished - 2005 Aug 15

Keywords

  • Photoaffinity labelling
  • Potassium lespedezate
  • Receptor

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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