Detection of β-1,2-mannosyltransferase in Candida albicans cells

Akifumi Suzuki, Yuka Takata, Asayo Oshie, Atsuko Tezuka, Nobuyuki Shibata, Hidemitsu Kobayashi, Yoshio Okawa, Shigeo Suzuki

Research output: Contribution to journalArticlepeer-review

25 Citations (Scopus)

Abstract

A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.

Original languageEnglish
Pages (from-to)275-279
Number of pages5
JournalFEBS Letters
Volume373
Issue number3
DOIs
Publication statusPublished - 1995 Oct 16
Externally publishedYes

Keywords

  • Candida albicans
  • Mannosyltransferase
  • β-1,2-Linked mannose

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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