Abstract
A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.
Original language | English |
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Pages (from-to) | 275-279 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 373 |
Issue number | 3 |
DOIs | |
Publication status | Published - 1995 Oct 16 |
Keywords
- Candida albicans
- Mannosyltransferase
- β-1,2-Linked mannose
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology