TY - JOUR
T1 - Detection of β-1,2-mannosyltransferase in Candida albicans cells
AU - Suzuki, Akifumi
AU - Takata, Yuka
AU - Oshie, Asayo
AU - Tezuka, Atsuko
AU - Shibata, Nobuyuki
AU - Kobayashi, Hidemitsu
AU - Okawa, Yoshio
AU - Suzuki, Shigeo
N1 - Copyright:
Copyright 2015 Elsevier B.V., All rights reserved.
PY - 1995/10/16
Y1 - 1995/10/16
N2 - A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.
AB - A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.
KW - Candida albicans
KW - Mannosyltransferase
KW - β-1,2-Linked mannose
UR - http://www.scopus.com/inward/record.url?scp=0028970848&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028970848&partnerID=8YFLogxK
U2 - 10.1016/0014-5793(95)01061-I
DO - 10.1016/0014-5793(95)01061-I
M3 - Article
C2 - 7589482
AN - SCOPUS:0028970848
VL - 373
SP - 275
EP - 279
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -