Detection of β-1,2-mannosyltransferase in Candida albicans cells

Akifumi Suzuki; Yuka Takata; Asayo Oshie; Atsuko Tezuka; Nobuyuki Shibata; Hidemitsu Kobayashi; Yoshio Okawa; Shigeo Suzuki

doi:10.1016/0014-5793(95)01061-I

Detection of β-1,2-mannosyltransferase in Candida albicans cells

Akifumi Suzuki, Yuka Takata, Asayo Oshie, Atsuko Tezuka, Nobuyuki Shibata, Hidemitsu Kobayashi, Yoshio Okawa, Shigeo Suzuki

Clinical Research, Innovation and Education Center

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)₂2Man (αβMan₅), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by ¹H NMR indicates that αβMan₅ was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)₂2Man (αβMan₆). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn²⁺ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan₅ and αβMan₆ side chains, also exhibited the same β-1,2-ManTase II activity.

Original language	English
Pages (from-to)	275-279
Number of pages	5
Journal	FEBS Letters
Volume	373
Issue number	3
DOIs	https://doi.org/10.1016/0014-5793(95)01061-I
Publication status	Published - 1995 Oct 16

Keywords

Candida albicans
Mannosyltransferase
β-1,2-Linked mannose

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(95)01061-I

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title = "Detection of β-1,2-mannosyltransferase in Candida albicans cells",

abstract = "A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.",

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AU - Suzuki, Akifumi

AU - Takata, Yuka

AU - Oshie, Asayo

AU - Tezuka, Atsuko

AU - Shibata, Nobuyuki

AU - Kobayashi, Hidemitsu

AU - Okawa, Yoshio

AU - Suzuki, Shigeo

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Y1 - 1995/10/16

N2 - A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.

AB - A particulate insoluble fraction from Candida albicans J-1012 (serotype A) strain cells was obtained as the residue after extracting a 105,000 × g pellet of cell homogenate with 1% Triton X-100. Incubation of this fraction with a mannopentaose, Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan5), in the presence of GDP-mannose followed by high performance liquid chromatography showed the formation of a mannohexaose. Analysis of the product by 1H NMR indicates that αβMan5 was changed to Manβ1 → 2Manβ1 → 2Manα1 → (2Manα1 →)22Man (αβMan6). This β-1,2-mannosyltransferase (ManTase) II activity was completely inhibited by Zn2+ and was not restored by the addition of EDTA. The corresponding enzyme fraction from C. albicans NIH B-792 (serotype B) strain cells, the mannan of which does not possess both the αβMan5 and αβMan6 side chains, also exhibited the same β-1,2-ManTase II activity.

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KW - β-1,2-Linked mannose

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Detection of β-1,2-mannosyltransferase in Candida albicans cells

Abstract

Keywords

ASJC Scopus subject areas

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