Detection frequencies and the colony-forming unit recovery of oral treponemes by different cultivation methods

T. Koseki, Y. Benno, Y. J. Zhang-Koseki, M. Umeda, I. Ishikawa

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Selected media were compared for primary isolation and detection of oral treponemes from clinical samples. Forty-eight subgingival plaque samples from 45 patients suffering from periodontitis were anaerobically cultivated for 2 weeks at 37°C. Of the 9 media studied, Medium 10 (M 10), which was supplemented with 10% rabbit serum and incubated using the plate-in-bottle method, supported the highest colony-forming units of the anaerobes. The treponemal colonies were detected at least on one medium from 83% of the subgingival plaque samples. The new oral spirochete medium in an anaerobic chamber supported the highest detection frequency of the oral treponemes (64% of samples); however, M 10 in the plate-in-bottle was found to produce the highest colony-forming unit recovery of the oral treponemes (median 3.6% of the total colony-forming units). This study suggests that M 10 in the plate- in-bottle and new oral spirochete medium in the anaerobic chamber are essential in cultivating oral treponemes.

Original languageEnglish
Pages (from-to)203-208
Number of pages6
JournalOral Microbiology and Immunology
Volume11
Issue number3
DOIs
Publication statusPublished - 1996 Jan 1
Externally publishedYes

Keywords

  • Medium 10
  • anaerobic culture
  • new oral spirochete medium
  • oral spirochete

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Dentistry(all)
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Detection frequencies and the colony-forming unit recovery of oral treponemes by different cultivation methods'. Together they form a unique fingerprint.

  • Cite this