Detection and time-course analysis of phospholipid hydroperoxide in soybean seedlings after treatment with fungal elicitor, by chemiluminescence-HPLC assay

Yasuo Kondo, Teruo Miyazawa, Junya Mizutani

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Direct evidence for the existence of phosphatidylcholine hydroperoxide (PCOOH) and the determination of the changes in PCOOH after treatment of soybean seedlings with fungal elicitor, were examined using chemiluminescence-HPLC of hydroperoxide specific assay. PCOOH was transiently accumulated within 6 h after elicitor treatment but such an event was not observed in control plants, thus suggesting that accumulation of PCOOH was a response to fungal elicitor in the host plant. The result suggested that singlet oxygen had participated in the lipid hydroperoxidation, since non-conjugated fatty acid hydroperoxide isomers due to the singlet oxygen oxidation were detected in the phospholipid fraction. The reaction of lipid hydroperoxidation, precedding the induction of a defense compound, may be involved in the triggering of a plant defense mechanism against fungal invasion.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalBiochimica et Biophysica Acta (BBA)/Lipids and Lipid Metabolism
Volume1127
Issue number3
DOIs
Publication statusPublished - 1992 Aug 19

Keywords

  • (Soybean)
  • Chemiluminescence-HPLC
  • Hydroperoxide specific assay
  • Phosphatidylcholine hydroperoxide
  • Phytoalexin induction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Endocrinology

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