Detection and molecular characterization of Cryptosporidium and Eimeria species in Philippine bats

Fumi Murakoshi, Frances C. Recuenco, Tsutomu Omatsu, Kaori Sano, Satoshi Taniguchi, Joseph S. Masangkay, Philip Alviola, Eduardo Eres, Edison Cosico, James Alvarez, Yumi Une, Shigeru Kyuwa, Yuki Sugiura, Kentaro Kato

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

The genus Cryptosporidium, which is an obligate intracellular parasite, infects various vertebrates and causes a diarrheal disease known as cryptosporidiosis. Bats are naturally infected with zoonotic pathogens; thus, they are potential reservoirs of parasites. We investigated the species and genotype distribution as well as prevalence of Cryptosporidium and Eimeria in Philippine bats. We captured and examined 45 bats; four were positive for Cryptosporidium spp. and seven were positive for Eimeria spp. We detected Cryptosporidium bat genotype II from Ptenochirus jagori. Three other Cryptosporidium sequences, detected from Rhinolophus inops, Cynopterus brachyotis, and Eonycteris spelaea, could not be classified as any known species or genotype; we therefore propose the novel genotype Cryptosporidium bat genotypes V, VI, and VII. Bat genotype V is associated with human cryptosporidiosis clade, and therefore, this genotype may be transmissible to humans. Among the Eimeria sequences, BE3 detected from Scotophilus kuhlii was classified with known bat and rodent clades; however, other sequences detected from C. brachyotis, E. spelaea, Rousettus amplexicaudatus, and R. inops could not be classified with known Eimeria species. These isolates might represent a new genotype. Our findings demonstrate that the bats of the Philippines represent a reservoir of multiple Cryptosporidium and Eimeria spp.

Original languageEnglish
Pages (from-to)1863-1869
Number of pages7
JournalParasitology Research
Volume115
Issue number5
DOIs
Publication statusPublished - 2016 May 1
Externally publishedYes

Keywords

  • Bat
  • Cryptosporidium
  • Cryptosporidium bat genotype
  • Eimeria

ASJC Scopus subject areas

  • Parasitology
  • veterinary(all)
  • Insect Science
  • Infectious Diseases

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