Detection and cloning of an X-linked locus associated with a NotI site that is not methylated on mouse inactivated X chromosome by the RLGS-M method

Shuji Takada, Mamoru Kamiya, Takahiro Arima, Hisashi Kagebayashi, Hideo Shibata, Masami Muramatsu, Verne M. Chapman, Norio Wake, Yoshihide Hayashizaki, Nobuo Takagi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In looking for genes that escape X chromosome inactivation, we scanned the methylation status of genomic DNA from XX, X0, and XY mice using the method of restriction landmark genomic scanning using methylation-sensitive endonuclease. We detected and cloned a candidate locus and identified the Orf1 gene. Orf1 has sequence similarities to the B2 repetitive element and human CXORF4 (formerly called EXLM1), which escapes X inactivation. The B2 element spans the 3' terminus of the ORF and the 3' UTR of Orf1. The Orf1 gene encompasses 18.5 kb of genomic DNA including 11 exons and 10 introns. Taking advantage of genomic polymorphisms present between MSM and C3H/He, we showed that murine Orf1 is mapped to the proximal region of the X chromosome. Despite the unmethylation of the NotI site, Orf1 is subject to X inactivation.

Original languageEnglish
Pages (from-to)92-100
Number of pages9
JournalGenomics
Volume61
Issue number1
DOIs
Publication statusPublished - 1999 Oct 1
Externally publishedYes

ASJC Scopus subject areas

  • Genetics

Fingerprint Dive into the research topics of 'Detection and cloning of an X-linked locus associated with a NotI site that is not methylated on mouse inactivated X chromosome by the RLGS-M method'. Together they form a unique fingerprint.

  • Cite this

    Takada, S., Kamiya, M., Arima, T., Kagebayashi, H., Shibata, H., Muramatsu, M., Chapman, V. M., Wake, N., Hayashizaki, Y., & Takagi, N. (1999). Detection and cloning of an X-linked locus associated with a NotI site that is not methylated on mouse inactivated X chromosome by the RLGS-M method. Genomics, 61(1), 92-100. https://doi.org/10.1006/geno.1999.5944