Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore

Tomoki Iwama; Mayo Komatsu; Kumi Y. Inoue; Hitoshi Shiku

doi:10.1002/celc.202100675

Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore

Tomoki Iwama, Mayo Komatsu, Kumi Y. Inoue, Hitoshi Shiku

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A 2D imaging system using closed bipolar electrodes (cBPEs) and electrochemiluminescence (ECL) is expected to realize high spatio-temporal resolution imaging. However, imaging of the distributions of molecules that cause an oxidation reaction on the electrode surface was not achieved by the cBPE/ECL system because the conventionally used luminophore can generate ECL only by an oxidation reaction. In this paper, we propose to implement a cathodic luminophore, [Ru(bpy)₃]Cl₂/glutathione disulfide (GSSG) producing ECL by reduction reaction into the cBPE/ECL system to enable detection and 2D imaging of dopamine, which is an oxidizable redox species and an important neurotransmitter. The applicability of [Ru(bpy)₃]Cl₂/GSSG for dopamine detection using a single cBPE was shown and its electrochemical properties were discussed. In addition, the demonstration of the 2D imaging of dopamine was performed using a cBPE array. These results show the potential to greatly expand the applicability of detection and imaging systems using cBPE/ECL.

Original language	English
Pages (from-to)	3492-3498
Number of pages	7
Journal	ChemElectroChem
Volume	8
Issue number	18
DOIs	https://doi.org/10.1002/celc.202100675
Publication status	Published - 2021 Sep 13

Keywords

2D imaging
bipolar electrochemical microscopy
bipolar electrochemistry
cathodic electrochemiluminescnece
dopamine

ASJC Scopus subject areas

Catalysis
Electrochemistry

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10.1002/celc.202100675

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title = "Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore",

abstract = "A 2D imaging system using closed bipolar electrodes (cBPEs) and electrochemiluminescence (ECL) is expected to realize high spatio-temporal resolution imaging. However, imaging of the distributions of molecules that cause an oxidation reaction on the electrode surface was not achieved by the cBPE/ECL system because the conventionally used luminophore can generate ECL only by an oxidation reaction. In this paper, we propose to implement a cathodic luminophore, [Ru(bpy)3]Cl2/glutathione disulfide (GSSG) producing ECL by reduction reaction into the cBPE/ECL system to enable detection and 2D imaging of dopamine, which is an oxidizable redox species and an important neurotransmitter. The applicability of [Ru(bpy)3]Cl2/GSSG for dopamine detection using a single cBPE was shown and its electrochemical properties were discussed. In addition, the demonstration of the 2D imaging of dopamine was performed using a cBPE array. These results show the potential to greatly expand the applicability of detection and imaging systems using cBPE/ECL.",

keywords = "2D imaging, bipolar electrochemical microscopy, bipolar electrochemistry, cathodic electrochemiluminescnece, dopamine",

author = "Tomoki Iwama and Mayo Komatsu and Inoue, {Kumi Y.} and Hitoshi Shiku",

note = "Funding Information: This research was supported by Grant‐in‐Aid for JSPS Fellows (no. 19J20709) from the Japan Society for the Promotion of Science (JSPS), Grant‐in‐Aid for Scientific Research (C) (no. 19K05687). It was also partially supported by JST COI (no. JPMJCE1303) from the Japan Science and Technology Agency (JST). Publisher Copyright: {\textcopyright} 2021 Wiley-VCH GmbH",

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doi = "10.1002/celc.202100675",

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AU - Komatsu, Mayo

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AU - Shiku, Hitoshi

N1 - Funding Information: This research was supported by Grant‐in‐Aid for JSPS Fellows (no. 19J20709) from the Japan Society for the Promotion of Science (JSPS), Grant‐in‐Aid for Scientific Research (C) (no. 19K05687). It was also partially supported by JST COI (no. JPMJCE1303) from the Japan Science and Technology Agency (JST). Publisher Copyright: © 2021 Wiley-VCH GmbH

PY - 2021/9/13

Y1 - 2021/9/13

N2 - A 2D imaging system using closed bipolar electrodes (cBPEs) and electrochemiluminescence (ECL) is expected to realize high spatio-temporal resolution imaging. However, imaging of the distributions of molecules that cause an oxidation reaction on the electrode surface was not achieved by the cBPE/ECL system because the conventionally used luminophore can generate ECL only by an oxidation reaction. In this paper, we propose to implement a cathodic luminophore, [Ru(bpy)3]Cl2/glutathione disulfide (GSSG) producing ECL by reduction reaction into the cBPE/ECL system to enable detection and 2D imaging of dopamine, which is an oxidizable redox species and an important neurotransmitter. The applicability of [Ru(bpy)3]Cl2/GSSG for dopamine detection using a single cBPE was shown and its electrochemical properties were discussed. In addition, the demonstration of the 2D imaging of dopamine was performed using a cBPE array. These results show the potential to greatly expand the applicability of detection and imaging systems using cBPE/ECL.

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Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore

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