Detecting Differences of Fluorescent Markers Distribution in Single Cell Microscopy: Textural or Pointillist Feature Space?

Ali Ahmad, Carole Frindel, David Rousseau

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


We consider the detection of change in spatial distribution of fluorescent markers inside cells imaged by single cell microscopy. Such problems are important in bioimaging since the density of these markers can reflect the healthy or pathological state of cells, the spatial organization of DNA, or cell cycle stage. With the new super-resolved microscopes and associated microfluidic devices, bio-markers can be detected in single cells individually or collectively as a texture depending on the quality of the microscope impulse response. In this work, we propose, via numerical simulations, to address detection of changes in spatial density or in spatial clustering with an individual (pointillist) or collective (textural) approach by comparing their performances according to the size of the impulse response of the microscope. Pointillist approaches show good performances for small impulse response sizes only, while all textural approaches are found to overcome pointillist approaches with small as well as with large impulse response sizes. These results are validated with real fluorescence microscopy images with conventional resolution. This, a priori non-intuitive result in the perspective of the quest of super-resolution, demonstrates that, for difference detection tasks in single cell microscopy, super-resolved microscopes may not be mandatory and that lower cost, sub-resolved, microscopes can be sufficient.

Original languageEnglish
Article number39
JournalFrontiers in Robotics and AI
Publication statusPublished - 2020 May 22
Externally publishedYes


  • classification
  • fluorescence
  • microscopy
  • point spread function
  • spot detection
  • texture

ASJC Scopus subject areas

  • Computer Science Applications
  • Artificial Intelligence


Dive into the research topics of 'Detecting Differences of Fluorescent Markers Distribution in Single Cell Microscopy: Textural or Pointillist Feature Space?'. Together they form a unique fingerprint.

Cite this