Designed hapten aimed at anti-ciguatoxin monoclonal antibody: Synthesis, immunization and discrimination of the C2 configuration

Hiroki Oguri, Shin Ichiro Tanaka, Shojiro Hishiyama, Tohru Oishi, Masahiro Hirama, Takeshi Tumuraya, Yoshihisa Tomioka, Michinao Mizugaki

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

The ABC-ring fragment of ciguatoxin was synthesized as a C2 epimeric mixture (1:1). The mixture, which was designed as a possible hapten for preparing an anti-ciguatoxin monoclonal antibody was conjugated with a carrier protein (KLH) and then used for immunization of mice. The three monoctonal antibodies (mAbs) obtained have shown an appreciable reactivity to the hapten. Each mAb discriminated configuration of the C2 hydroxy group of the ABC-ring fragment. The mAb which showed reactivity to the 2S-fragment was cross-reacted with ciguatoxin.

Original languageEnglish
Pages (from-to)1431-1436
Number of pages6
JournalSynthesis
Issue numberSPEC. ISS.
Publication statusPublished - 1999 Jan 1

Keywords

  • Anti-ciguatoxin antibody
  • Artificial antigen
  • Ciguatoxin
  • Immunization
  • Synthetic hapten

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

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    Oguri, H., Tanaka, S. I., Hishiyama, S., Oishi, T., Hirama, M., Tumuraya, T., Tomioka, Y., & Mizugaki, M. (1999). Designed hapten aimed at anti-ciguatoxin monoclonal antibody: Synthesis, immunization and discrimination of the C2 configuration. Synthesis, (SPEC. ISS.), 1431-1436.