TY - JOUR
T1 - Design, preparation and assessment of surface-immobilised tetraphenylethenes for biosensing applications
AU - Brennecke, Johannes
AU - Ochs, Christopher J.
AU - Boudhar, Aïcha
AU - Reux, Bastien
AU - Subramanian, Gomathy Sandhya
AU - Lear, Martin J.
AU - Trau, Dieter
AU - Hobley, Jonathan
N1 - Funding Information:
The authors thank the National University of Singapore (NUS) for funding ( R397000154112 and R397000150592 ). This research was also supported by the National Research Foundation Singapore (SMART Scholars fund S900074) through the Singapore MIT Alliance for Research and Technology's BioSystems and Micromechanics Inter-Disciplinary Research programme. Jonathan Hobley is grateful to A-STAR Joint Council Office for funding JCO Grant No 10/03/FG/06/04. Johannes Brennecke thanks the Studienstiftung des deutschen Volkes and the Agency for Science, Technology and Research (A*Star) for travel and internship support.
PY - 2014/7/15
Y1 - 2014/7/15
N2 - Tetraphenylethene (TPE) shows a significant increase of fluorescence intensity when the rotational freedom of its phenyl groups is restricted. This special property allows the use of TPE in sensor applications, which have been previously described for the liquid phase only. However, some applications utilising arrays require the immobilisation of TPE dyes on solid surfaces. In this work, we synthesised and investigated the fluorescence behaviour of TPE derivatives on silica particles and quartz slides and suggest ways to employ the dye's properties in solid phase biosensor applications.
AB - Tetraphenylethene (TPE) shows a significant increase of fluorescence intensity when the rotational freedom of its phenyl groups is restricted. This special property allows the use of TPE in sensor applications, which have been previously described for the liquid phase only. However, some applications utilising arrays require the immobilisation of TPE dyes on solid surfaces. In this work, we synthesised and investigated the fluorescence behaviour of TPE derivatives on silica particles and quartz slides and suggest ways to employ the dye's properties in solid phase biosensor applications.
KW - Bioassay
KW - Fluorescent dye
KW - Microparticles
KW - Reagentless assay
KW - Tetraphenylethene
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U2 - 10.1016/j.apsusc.2014.04.061
DO - 10.1016/j.apsusc.2014.04.061
M3 - Article
AN - SCOPUS:84901348119
VL - 307
SP - 475
EP - 481
JO - Applied Surface Science
JF - Applied Surface Science
SN - 0169-4332
ER -