Design of stimuli-responsive nano boxes with chaperonins

Hisae Kuwagaki; Kazushi Kinbara; Hideki Taguchi; Masasuke Yoshida; Takuzo Aida

Design of stimuli-responsive nano boxes with chaperonins

Hisae Kuwagaki, Kazushi Kinbara, Hideki Taguchi, Masasuke Yoshida, Takuzo Aida

Institute of Multidisciplinary Research for Advanced Materials (IMRAM)

Research output: Contribution to conference › Paper › peer-review

Abstract

Chaperonin GroEL undergoes a large conformational change in response to ATP. We prepared new mutant GroELs, CA-R231C and CA-K311C+L314C, where cystein residues exist around the entrance the cavity. Fluorescent units were introduced to the mutants through the reaction of the thiol of cystein group with maleimide group of the fluorescent molecule. The optical properties of thus modified GroELs will be reported.

Original language	English
Pages	4959-4960
Number of pages	2
Publication status	Published - 2005 Dec 1
Event	54th SPSJ Symposium on Macromolecules - Yamagata, Japan Duration: 2005 Sep 20 → 2005 Sep 22

Other

Other	54th SPSJ Symposium on Macromolecules
Country/Territory	Japan
City	Yamagata
Period	05/9/20 → 05/9/22

Keywords

ATPase
Chaperonin
Fluorescent molecule

ASJC Scopus subject areas

Engineering(all)

Cite this

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title = "Design of stimuli-responsive nano boxes with chaperonins",

abstract = "Chaperonin GroEL undergoes a large conformational change in response to ATP. We prepared new mutant GroELs, CA-R231C and CA-K311C+L314C, where cystein residues exist around the entrance the cavity. Fluorescent units were introduced to the mutants through the reaction of the thiol of cystein group with maleimide group of the fluorescent molecule. The optical properties of thus modified GroELs will be reported.",

keywords = "ATPase, Chaperonin, Fluorescent molecule",

author = "Hisae Kuwagaki and Kazushi Kinbara and Hideki Taguchi and Masasuke Yoshida and Takuzo Aida",

year = "2005",

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day = "1",

language = "English",

pages = "4959--4960",

note = "54th SPSJ Symposium on Macromolecules ; Conference date: 20-09-2005 Through 22-09-2005",

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TY - CONF

T1 - Design of stimuli-responsive nano boxes with chaperonins

AU - Kuwagaki, Hisae

AU - Kinbara, Kazushi

AU - Taguchi, Hideki

AU - Yoshida, Masasuke

AU - Aida, Takuzo

PY - 2005/12/1

Y1 - 2005/12/1

N2 - Chaperonin GroEL undergoes a large conformational change in response to ATP. We prepared new mutant GroELs, CA-R231C and CA-K311C+L314C, where cystein residues exist around the entrance the cavity. Fluorescent units were introduced to the mutants through the reaction of the thiol of cystein group with maleimide group of the fluorescent molecule. The optical properties of thus modified GroELs will be reported.

AB - Chaperonin GroEL undergoes a large conformational change in response to ATP. We prepared new mutant GroELs, CA-R231C and CA-K311C+L314C, where cystein residues exist around the entrance the cavity. Fluorescent units were introduced to the mutants through the reaction of the thiol of cystein group with maleimide group of the fluorescent molecule. The optical properties of thus modified GroELs will be reported.

KW - ATPase

KW - Chaperonin

KW - Fluorescent molecule

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UR - http://www.scopus.com/inward/citedby.url?scp=33645567779&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:33645567779

SP - 4959

EP - 4960

T2 - 54th SPSJ Symposium on Macromolecules

Y2 - 20 September 2005 through 22 September 2005

ER -

Design of stimuli-responsive nano boxes with chaperonins

Abstract

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Keywords

ASJC Scopus subject areas

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