Design of nanogel crosslinked hydrogels and function as artificial molecular chaperone

Nobuyuki Morimoto; Kazunari Akiyoshi

Design of nanogel crosslinked hydrogels and function as artificial molecular chaperone

Research output: Contribution to conference › Paper › peer-review

Abstract

We develop tailor-made functional nanogels to create novel nanobiomaterials (nanogel engineering) by self-assembling of functional associating polymers as building blocks. Self-assembled nanogels containing polymerizable groups (CHPMA) were designed as key building blocks. Hybrid hydrogels were prepared by copolymerization of the CHPMA with 2-methacryloyloxyethyl phosphorylcholine (MPC) in semi-dilute aqueous condition. The hydrogels trapped proteins in denatured form and rapidly released by adding ß-CD solution. The released proteins were refolded as native form. The hydrogels worked well as an artificial molecular chaperone system for protein refolding.

Original language	English
Pages	4522-4523
Number of pages	2
Publication status	Published - 2006 Dec 1
Externally published	Yes
Event	55th Society of Polymer Science Japan Symposium on Macromolecules - Toyama, Japan Duration: 2006 Sep 20 → 2006 Sep 22

Other

Other	55th Society of Polymer Science Japan Symposium on Macromolecules
Country	Japan
City	Toyama
Period	06/9/20 → 06/9/22

Keywords

2-Methacryloyloxyethyl phosphorylcholine
Artificial molecular chaperone
Cholesteryl group bearing pullulan
Hydrogel
Nanogel

ASJC Scopus subject areas

Engineering(all)

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title = "Design of nanogel crosslinked hydrogels and function as artificial molecular chaperone",

abstract = "We develop tailor-made functional nanogels to create novel nanobiomaterials (nanogel engineering) by self-assembling of functional associating polymers as building blocks. Self-assembled nanogels containing polymerizable groups (CHPMA) were designed as key building blocks. Hybrid hydrogels were prepared by copolymerization of the CHPMA with 2-methacryloyloxyethyl phosphorylcholine (MPC) in semi-dilute aqueous condition. The hydrogels trapped proteins in denatured form and rapidly released by adding {\ss}-CD solution. The released proteins were refolded as native form. The hydrogels worked well as an artificial molecular chaperone system for protein refolding.",

keywords = "2-Methacryloyloxyethyl phosphorylcholine, Artificial molecular chaperone, Cholesteryl group bearing pullulan, Hydrogel, Nanogel",

author = "Nobuyuki Morimoto and Kazunari Akiyoshi",

year = "2006",

month = dec,

day = "1",

language = "English",

pages = "4522--4523",

note = "55th Society of Polymer Science Japan Symposium on Macromolecules ; Conference date: 20-09-2006 Through 22-09-2006",

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TY - CONF

T1 - Design of nanogel crosslinked hydrogels and function as artificial molecular chaperone

AU - Morimoto, Nobuyuki

AU - Akiyoshi, Kazunari

PY - 2006/12/1

Y1 - 2006/12/1

N2 - We develop tailor-made functional nanogels to create novel nanobiomaterials (nanogel engineering) by self-assembling of functional associating polymers as building blocks. Self-assembled nanogels containing polymerizable groups (CHPMA) were designed as key building blocks. Hybrid hydrogels were prepared by copolymerization of the CHPMA with 2-methacryloyloxyethyl phosphorylcholine (MPC) in semi-dilute aqueous condition. The hydrogels trapped proteins in denatured form and rapidly released by adding ß-CD solution. The released proteins were refolded as native form. The hydrogels worked well as an artificial molecular chaperone system for protein refolding.

AB - We develop tailor-made functional nanogels to create novel nanobiomaterials (nanogel engineering) by self-assembling of functional associating polymers as building blocks. Self-assembled nanogels containing polymerizable groups (CHPMA) were designed as key building blocks. Hybrid hydrogels were prepared by copolymerization of the CHPMA with 2-methacryloyloxyethyl phosphorylcholine (MPC) in semi-dilute aqueous condition. The hydrogels trapped proteins in denatured form and rapidly released by adding ß-CD solution. The released proteins were refolded as native form. The hydrogels worked well as an artificial molecular chaperone system for protein refolding.

KW - 2-Methacryloyloxyethyl phosphorylcholine

KW - Artificial molecular chaperone

KW - Cholesteryl group bearing pullulan

KW - Hydrogel

KW - Nanogel

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M3 - Paper

AN - SCOPUS:33846128434

SP - 4522

EP - 4523

T2 - 55th Society of Polymer Science Japan Symposium on Macromolecules

Y2 - 20 September 2006 through 22 September 2006

ER -

Design of nanogel crosslinked hydrogels and function as artificial molecular chaperone

Abstract

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Keywords

ASJC Scopus subject areas

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