Design and synthesis of a second-generation ligand-tethered calcium indicator for plant cell biology based on the fundamental analyses of the structure and physical property

Yousuke Takaoka, Masaki Imai, Miyuki Shigenaga, Minoru Ueda

Research output: Contribution to journalArticlepeer-review

Abstract

Calcium ion (Ca2+) is one of the most important second messengers. However, conventional Ca2+ indicators cannot be applied in plant cells. In previous paper, we developed the first-generation protein-ligand tethered calcium indicator that can be applied for living plant cells. Here, we will report the development of second-generation indicator based on the detailed examination on physical properties of the first-generation to improve the drawbacks. Through the examination, we also found that cell permeability of the probe is strongly affected by their aggregation properties. Our findings will provide new strategy for the design of synthetic indicators.

Original languageEnglish
Pages (from-to)3079-3085
Number of pages7
JournalTetrahedron
Volume73
Issue number21
DOIs
Publication statusPublished - 2017

Keywords

  • Calcium probe
  • Chemical biology
  • Plant cell

ASJC Scopus subject areas

  • Biochemistry
  • Drug Discovery
  • Organic Chemistry

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