Design and optimization of fermentation medium for enhanced bacteriocin production by probiotic bacterium enterococcus faecium MC13

Paulraj Kanmani, R. Satish Kumar, N. Yuvaraj, K. A. Paari, V. Pattukumar, Venkatesan Arul

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    Statistics-based experimental designs were used to develop a cost-effective medium for enhanced production of viable cells and bacteriocin by probiotic Enterococcus faecium MC13. Carbon, nitrogen, and mineral sources were first screened by one-variable-at-a-time (OVAT) methods. In order to increase yield production, the selected variables were further statistically optimized using response-surface methodology (RSM) with central composite design (CCD). The maximum and minimum levels of the selected variables were determined and a set of 34 experimental runs was performed. The optimum concentrations of the tested variables for production of viable cells (12.24 log CFU mL-1) and bacteriocin activity (25,600AU mL-1) were tryptone (10.0g/L), peptone (6.0g/L), maltose (3.0g/L), glucose (9.0g/L), NaCl (15.0g/L), sodium citrate (2.5g/L), sodium acetate (1.0g/L), and dipotassium PO4 (0.1g/L). Threefold increased yield of bacteriocin was achieved in optimized medium compared to the unoptimized counterpart, and this was two times less cost than commercial MRS medium.

    Original languageEnglish
    Pages (from-to)40-52
    Number of pages13
    JournalPreparative Biochemistry and Biotechnology
    Volume41
    Issue number1
    DOIs
    Publication statusPublished - 2011 Jan

    Keywords

    • Enterococcus faecium MC13
    • bacteriocin
    • optimization
    • response-surface methodology
    • total viable cells

    ASJC Scopus subject areas

    • Biotechnology
    • Biochemistry

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