TY - JOUR
T1 - Design and optimization of fermentation medium for enhanced bacteriocin production by probiotic bacterium enterococcus faecium MC13
AU - Kanmani, Paulraj
AU - Kumar, R. Satish
AU - Yuvaraj, N.
AU - Paari, K. A.
AU - Pattukumar, V.
AU - Arul, Venkatesan
N1 - Funding Information:
This work was supported by a grant from the Department of Biotechnology, New Delhi.
PY - 2011/1
Y1 - 2011/1
N2 - Statistics-based experimental designs were used to develop a cost-effective medium for enhanced production of viable cells and bacteriocin by probiotic Enterococcus faecium MC13. Carbon, nitrogen, and mineral sources were first screened by one-variable-at-a-time (OVAT) methods. In order to increase yield production, the selected variables were further statistically optimized using response-surface methodology (RSM) with central composite design (CCD). The maximum and minimum levels of the selected variables were determined and a set of 34 experimental runs was performed. The optimum concentrations of the tested variables for production of viable cells (12.24 log CFU mL-1) and bacteriocin activity (25,600AU mL-1) were tryptone (10.0g/L), peptone (6.0g/L), maltose (3.0g/L), glucose (9.0g/L), NaCl (15.0g/L), sodium citrate (2.5g/L), sodium acetate (1.0g/L), and dipotassium PO4 (0.1g/L). Threefold increased yield of bacteriocin was achieved in optimized medium compared to the unoptimized counterpart, and this was two times less cost than commercial MRS medium.
AB - Statistics-based experimental designs were used to develop a cost-effective medium for enhanced production of viable cells and bacteriocin by probiotic Enterococcus faecium MC13. Carbon, nitrogen, and mineral sources were first screened by one-variable-at-a-time (OVAT) methods. In order to increase yield production, the selected variables were further statistically optimized using response-surface methodology (RSM) with central composite design (CCD). The maximum and minimum levels of the selected variables were determined and a set of 34 experimental runs was performed. The optimum concentrations of the tested variables for production of viable cells (12.24 log CFU mL-1) and bacteriocin activity (25,600AU mL-1) were tryptone (10.0g/L), peptone (6.0g/L), maltose (3.0g/L), glucose (9.0g/L), NaCl (15.0g/L), sodium citrate (2.5g/L), sodium acetate (1.0g/L), and dipotassium PO4 (0.1g/L). Threefold increased yield of bacteriocin was achieved in optimized medium compared to the unoptimized counterpart, and this was two times less cost than commercial MRS medium.
KW - Enterococcus faecium MC13
KW - bacteriocin
KW - optimization
KW - response-surface methodology
KW - total viable cells
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U2 - 10.1080/10826068.2010.534221
DO - 10.1080/10826068.2010.534221
M3 - Article
C2 - 21229463
AN - SCOPUS:78651395774
VL - 41
SP - 40
EP - 52
JO - Preparative Biochemistry and Biotechnology
JF - Preparative Biochemistry and Biotechnology
SN - 1082-6068
IS - 1
ER -