Dermatan sulfate inhibits osteoclast formation by binding to receptor activator of NF-κB ligand

Kouhei Shinmyouzu, Tetsu Takahashi, Wataru Ariyoshi, Hisashi Ichimiya, Shin Kanzaki, Tatsuji Nishihara

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Dermatan sulfate (DS) is a major component of extracellular matrices in mammalian tissues. In the present study, DS demonstrated a high level of binding activity to receptor activator of NF-κB ligand (RANKL) and obstructed the binding of RANK to RANKL, determined using a quartz-crystal microbalance (QCM) technique. Further, when mouse bone marrow cells were cultured with RANKL and macrophage colony-stimulating factor, DS suppressed tartrate-resistant acid phosphatase-positive multinucleated cell formation in a dose-dependent manner. In addition, immunoblot analyses revealed that DS reduced the levels of phosphorylation of p38 mitogen-activated protein kinase and extracellular signal-regulated kinase protein in mouse osteoclast progenitor cells stimulated with RANKL. Together, these results indicate that DS regulates osteoclast formation through binding to RANKL and inhibition of signal transduction in osteoclast progenitor cells, suggesting that it has an important role in bone metabolism in pathological conditions.

Original languageEnglish
Pages (from-to)447-452
Number of pages6
JournalBiochemical and biophysical research communications
Volume354
Issue number2
DOIs
Publication statusPublished - 2007 Mar 9
Externally publishedYes

Keywords

  • Dermatan sulfate
  • Glycosaminoglycan
  • Macrophage colony-stimulating factor
  • Osteoclast
  • Quartz-crystal microbalance technique
  • Receptor activator of NF-κB ligand

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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