Deregulation of sucrose-controlled translation of a bZIP-type transcription factor results in sucrose accumulation in leaves

Sunil Kumar Thalor, Thomas Berberich, Sung Shin Lee, Seung Hwan Yang, Xu Jun Zhu, Ryozo Imai, Yoshihiro Takahashi, Tomonobu Kusano

    Research output: Contribution to journalArticlepeer-review

    25 Citations (Scopus)

    Abstract

    Sucrose is known to repress the translation of Arabidopsis thaliana AtbZIP11 transcript which encodes a protein belonging to the group of S (S - stands for small) basic region-leucine zipper (bZIP)-type transcription factor. This repression is called sucrose-induced repression of translation (SIRT). It is mediated through the sucrose-controlled upstream open reading frame (SC-uORF) found in the AtbZIP11 transcript. The SIRT is reported for 4 other genes belonging to the group of S bZIP in Arabidopsis. Tobacco tbz17 is phylogenetically closely related to AtbZIP11 and carries a putative SC-uORF in its 5′-leader region. Here we demonstrate that tbz17 exhibits SIRT mediated by its SC-uORF in a manner similar to genes belonging to the S bZIP group of the Arabidopsis genus. Furthermore, constitutive transgenic expression of tbz17 lacking its 5′-leader region containing the SC-uORF leads to production of tobacco plants with thicker leaves composed of enlarged cells with 3-4 times higher sucrose content compared to wild type plants. Our finding provides a novel strategy to generate plants with high sucrose content.

    Original languageEnglish
    Article numbere33111
    JournalPloS one
    Volume7
    Issue number3
    DOIs
    Publication statusPublished - 2012 Mar 22

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)
    • General

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