Denopamine stimulates alveolar fluid clearance via cystic fibrosis transmembrane conductance regulator in rat lungs

Xiu Gu, Zheng Wang, Jin Xu, Sumiko Maeda, Makoto Sugita, Motoyasu Sagawa, Hirohisa Toga, Tsutomu Sakuma

    Research output: Contribution to journalArticlepeer-review

    10 Citations (Scopus)

    Abstract

    Objective: The objective of this study was to test the hypothesis that cystic fibrosis transmembrane conductance regulator (CFTR) plays a role in β1-adrenergic agonist-stimulated alveolar fluid clearance. Methods: Isotonic 5% albumin solutions containing different pharmacological agents were instilled into the alveolar spaces of the isolated rat lungs. The lungs were inflated with 100% oxygen at an airway pressure of 7 cm H 2O and placed in a humidified incubator at 37°C. Alveolar fluid clearance was estimated by the progressive increase in the albumin concentration over 1 h. To test the hypothesis, we determined whether CFTR Cl- channel inhibitors (glibenclamide and CFTRinh-172) inhibited the effect of denopamine, a β1-adrenergic agonist, on stimulation of alveolar fluid clearance in the isolated rat lungs. Results: Denopamine increased alveolar fluid clearance in a dose-dependent manner. Atenolol, a β1-adrenergic antagonist, abolished the effects of denopamine on stimulation of alveolar fluid clearance. Although glibenclamide alone or CFTRinh-172 alone did not change basal alveolar fluid clearance, these CFTR inhibitors inhibited the effect of denopamine on alveolar fluid clearance. Conclusion: CFTR plays a role in β1-adrenergic agonist-stimulated alveolar fluid clearance in rat lungs.

    Original languageEnglish
    Pages (from-to)566-571
    Number of pages6
    JournalRespirology
    Volume11
    Issue number5
    DOIs
    Publication statusPublished - 2006 Sep 1

    Keywords

    • Alveolar fluid clearance
    • Beta-adrenergic agonist
    • Cystic fibrosis transmembrane conductance regulator
    • Glibenclamide
    • Pulmonary oedema

    ASJC Scopus subject areas

    • Pulmonary and Respiratory Medicine

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