Demonstration of rod and cone photoreceptors in the lamprey retina by freeze-replication and immunofluorescence

Makoto Ishikawa, Masashi Takao, Hiroshi Washioka, Fumio Tokunaga, Hiroshi Watanabe, Akira Tonosaki

Research output: Contribution to journalArticlepeer-review

44 Citations (Scopus)

Abstract

In common with other cyclostomata, the Japanese river lamprey (Lampetra japonica) has a retina consisting of distinct types of photoreceptor cells called long and short photoreceptor cells. After freeze-fracture, disc membranes of these photoreceptor cells were characterized in common by a homogeneous distribution of intramembrane particles on the protoplasmic fracture faces, in contrast to those of the myeloid bodies bearing scattering particles. Immunofluorescent examination was applied to the retina with monoclonal antibodies raised against bovine and chicken rhodopsins. Positive immunoreactivity was found to be limited to outer segments of the short cell, leaving the entire body of the long cell and all other components of the retina negative. The results suggest that the short cell is more closely related to a rod-type photoreceptor cell characterized by rhodopsin as its visual pigment.

Original languageEnglish
Pages (from-to)241-246
Number of pages6
JournalCell and Tissue Research
Volume249
Issue number2
DOIs
Publication statusPublished - 1987 Aug 1

Keywords

  • Freeze-fracturing
  • Immunocytochemistry
  • Lampetra japonica
  • Photoreceptor cells
  • Retina
  • Ultrastructure

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Cell Biology

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