Deltex/Dtx mediates NOTCH signaling in regulation of Bmp4 expression in cranial neural crest formation during avian development

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30 Citations (Scopus)

Abstract

NOTCH signaling plays a key role in cell fate determination in both vertebrates and invertebrates. It is well known that Su(H)/RBP-J is a major mediator of NOTCH signaling. In a previous study, it was shown that NOTCH signaling was involved in cranial neural crest formation in avian embryos. However, Su(H)/RBP-J activity did not appear to be required in this process. In this study, the Deltex/Dtx gene was focussed on as a potential mediator of NOTCH signaling in neural crest formation. At the time of neural crest formation, quail Deltex2 was expressed throughout the ectoderm. Misexpression of a dominant-negative form of Deltex in the ectoderm caused reduced expression of Slug, a neural crest marker. Dominant-negative Deltex expression reduced the expression of Bmp4, a neural crest inducer, whereas co-transfection of Bmp4 with dominant-negative Deltex rescued Slug expression. In parallel, Hairy2 expression in the epidermis was regulated by a Su(H)-dependent pathway. These results indicate that NOTCH signaling has dual functions mediated by either Su(H)or Deltex in the avian embryonic ectoderm.

Original languageEnglish
Pages (from-to)241-248
Number of pages8
JournalDevelopment Growth and Differentiation
Volume45
Issue number3
DOIs
Publication statusPublished - 2003 Jun

Keywords

  • Bmp4
  • Deltex
  • Neural crest
  • Notch
  • Slug

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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