Delivery of ribosome-inactivating protein toxin into cancer cells with shock waves

Tetsuya Kodama, Apostolos G. Doukas, Michael R. Hamblin

Research output: Contribution to journalArticlepeer-review

28 Citations (Scopus)

Abstract

We report on the use of shock waves delivered by a shock-tube to permeabilize cancer cells and potentiate the cytotoxicity of the type-1 ribosome-inactivating protein, saporin. We studied human colorectal cancer HT29 and ovarian cancer OVCAR-5 cells, and used two different cytotoxicity assays, colony formation and loss of mitochondrial activity. A single shock wave and saporin (10-9 M) produced significant toxicity not seen with either shock wave or drug alone. Increasing the number of shock waves up to five further increased cytotoxicity. Higher toxicity was seen with the clonogenic assay compared to MTT assay. Shock waves may have applications in promoting cytoplasmic delivery of toxins into cancer cells after intratumoral injection.

Original languageEnglish
Pages (from-to)69-75
Number of pages7
JournalCancer Letters
Volume189
Issue number1
DOIs
Publication statusPublished - 2003 Jan 10

Keywords

  • Cancer
  • Cytoplasmic delivery
  • Ribosome-inactivating protein
  • Saporin
  • Shock tube

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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