Delineating developmental and metabolic pathways in vivo by expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays

Rika Miki, Koji Kadota, Hidemasa Bono, Yosuke Mizuno, Yasuhiro Tomaru, Piero Carninci, Masayoshi Itoh, Kazuhiro Shibata, Jun Kawai, Hideaki Konno, Sachihiko Watanabe, Kenjiro Sato, Yumiko Tokusumi, Noriko Kikuchi, Yoshiyuki Ishii, Yohei Hamaguchi, Itaru Nishizuka, Hitoshi Goto, Hiroyuki Nitanda, Susumu SatomiAtsushi Yoshiki, Moriaki Kusakabe, Joseph L. DeRisi, Michael B. Eisen, Vishwnath R. Iyer, Patrick O. Brown, Masami Muramatsu, Hiroshi Shimada, Yasushi Okazaki, Yoshihide Hayashizaki

Research output: Contribution to journalArticle

189 Citations (Scopus)

Abstract

We have systematically characterized gene expression patterns in 49 adult and embryonic mouse tissues by using cDNA microarrays with 18,816 mouse cDNAs. Cluster analysis defined sets of genes that were expressed ubiquitously or in similar groups of tissues such as digestive organs and muscle. Clustering of expression profiles was observed in embryonic brain, postnatal cerebellum, and adult olfactory bulb, reflecting similarities in neurogenesis and remodeling. Finally, clustering genes coding for known enzymes into 78 metabolic pathways revealed a surprising coordination of expression within each pathway among different tissues. On the other hand, a more detailed examination of glycolysis revealed tissue-specific differences in profiles of key regulatory enzymes. Thus, by surveying global gene expression by using microarrays with a large number of elements, we provide insights into the commonality and diversity of pathways responsible for the development and maintenance of the mammalian body plan.

Original languageEnglish
Pages (from-to)2199-2204
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume98
Issue number5
DOIs
Publication statusPublished - 2001 Feb 27

ASJC Scopus subject areas

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    Miki, R., Kadota, K., Bono, H., Mizuno, Y., Tomaru, Y., Carninci, P., Itoh, M., Shibata, K., Kawai, J., Konno, H., Watanabe, S., Sato, K., Tokusumi, Y., Kikuchi, N., Ishii, Y., Hamaguchi, Y., Nishizuka, I., Goto, H., Nitanda, H., ... Hayashizaki, Y. (2001). Delineating developmental and metabolic pathways in vivo by expression profiling using the RIKEN set of 18,816 full-length enriched mouse cDNA arrays. Proceedings of the National Academy of Sciences of the United States of America, 98(5), 2199-2204. https://doi.org/10.1073/pnas.041605498