Deletion of the ubiquitin-conjugating enzyme Ubc2 confers resistance to methylmercury in budding yeast by promoting Whi2 degradation

Gi Wook Hwang, Fujio Mastuyama, Tsutomu Takahashi, Jin Yong Lee, Akira Naganuma

Research output: Contribution to journalLetter

8 Citations (Scopus)

Abstract

Ubiquitin-conjugating enzymes involved in sensitivity to methylmercury in yeast were identified by deletion analysis, which showed that Ubc2- or Ubp13-deficiency conferred resistance to methylmercury. Whi2, which was previously shown to be associated with increased methylmercury toxicity and is intracellularly degraded via the ubiquitin-proteasome system, was expressed at significantly lower levels in Ubc2-deficient yeast than in wild-type yeast. Ubc2/Whi2 double-deficient yeast showed neither an additive nor synergistic increase in methylmercury resistance. Our results indicate that Ubc2 may increase the sensitivity to methylmercury in yeast by inhibiting the proteasomal degradation of Whi2.

Original languageEnglish
Pages (from-to)301-303
Number of pages3
JournalJournal of Toxicological Sciences
Volume38
Issue number2
DOIs
Publication statusPublished - 2013 Apr 3

Keywords

  • Methylmercury
  • Toxicity
  • Ubiquitin-conjugating enzyme
  • Whi2
  • Yeast

ASJC Scopus subject areas

  • Toxicology

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