Deletion analysis of AGD1 reveals domains crucial for plasma membrane recruitment and function in root hair polarity

Cheol Min Yoo, Satoshi Naramoto, J. Alan Sparks, Bibi Rafeiza Khan, Jin Nakashima, Hiroo Fukuda, Elison B. Blancaflor

    Research output: Contribution to journalArticle

    9 Citations (Scopus)

    Abstract

    AGD1, a plant ACAP-type ADP-ribosylation factor-GTPase activating protein (ARF-GAP), functions in specifying root hair polarity in Arabidopsis thaliana. To better understand how AGD1 modulates root hair growth, we generated full-length and domain-deleted AGD1-green fluorescent protein (GFP) constructs, and followed their localization during root hair development. AGD1-GFP localized to the cytoplasm and was recruited to specific regions of the root hair plasma membrane (PM). Distinct PMAGD1-GFP signal was first detected along the site of root hair bulge formation. The construct continued tomark the PMat the root hair apical dome, but only during periods of reduced growth. During rapid tip growth, AGD1-GFP labeled the PM of the lateral flanks and dissipated from the apical-most PM. Deletion analysis and a single domain GFP fusion revealed that the pleckstrin homology (PH) domain is the minimal unit required for recruitment of AGD1 to the PM. Our results indicate that differential recruitment of AGD1 to specific PM domains is an essential component of the membrane trafficking machinery that facilitates root hair developmental phase transitions and responses to changes in the root microenvironment.

    Original languageEnglish
    Article numberjcs.203828
    JournalJournal of cell science
    Volume131
    Issue number2
    DOIs
    Publication statusPublished - 2018 Jan 1

    Keywords

    • ADP-ribosylation factor-GTPase activating protein
    • Arabidopsis thaliana
    • Plasma membrane
    • Polarity
    • Root hairs
    • Tip growth

    ASJC Scopus subject areas

    • Cell Biology

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