Degree of apoptosis induced by adenovirus-mediated transduction of p53 or p73α depends on the p53 status of glioma cells

Nobusada Shinoura, Yukiko Muramatsu, Akio Asai, Shuangyin Han, Akira Horii, Takaaki Kirino, Hirofumi Hamada

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

It has been reported that U-87MG glioma cells with wild-type p53 are resistant to p53 replacement gene therapy. As some gliomas harbor wild-type p53, it would be important to override the resistance mechanism due to wild-type p53 in glioma gene therapy. In this study, we transduced U-87MG cells or U251 glioma cells harboring mutated p53 with the p53 or p73α gene (a homologue of p53, that differently induces some p53-responsive genes) via adenovirus vectors (Advs) at same multiplicities of infection (MOIs) into respective cells (U-87MG: MOI 1000, U251: MOI 100), and evaluated the degree of apoptosis. The results demonstrate that the degree of apoptosis induced by Adv-mediated transduction of p53 in U-87MG cells was lower than that in U251 cells, whereas that induced by Adv-mediated transduction of p73α in U-87MG cells was higher than that in U251 cells. Bax expression in U-87MG and U251 cells induced by Adv-mediated transduction of p53 was almost the same as that of p73α. On the other hand, Adv-mediated transduction of p73α induced caspase-9 at higher levels than that of p53 in both cells. The results indicate that Adv-mediated transduction of p73α might be beneficial to overcome the resistance mechanism of glioma cells harboring wild-type p53. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)67-73
Number of pages7
JournalCancer Letters
Volume160
Issue number1
DOIs
Publication statusPublished - 2000 Nov 10

Keywords

  • Adenovirus
  • Apoptosis
  • Gene therapy
  • Glioma
  • p53
  • p73

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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